AssayMax Human Complement C1 ELISA Kit 补体C1酶联免疫试剂盒
Introduction Complement component C1 (C1) is a calcium-dependent serine protease complex with an approximate mass of 790 kDa and acts as the first component of the classical complement pathway. C1 is formed from the association of a recognition protein C1q and two catalytic subunits C1r and C1s respectively (1 - 2). The globular heads of the C1q bind to the Fc-fragment of IgM or IgG on the surface of a pathogen, resulting in the activation of C1r. The activated C1r is able to activate C1s which in turn activates C2 and C4, leading to the production of the C4b-C2a form of C3-convertase (3 - 4). C1 deficiency is generally associated with severe immune complex disease of systemic lupus erythematosus and glomerulonephritis (5 - 6).
Principle of the Assay The AssayMax Human Complement C1 ELISA kit is designed for detection of C1 in human plasma, serum, saliva, urine, milk, and cell culture samples. This assay employs a quantitative sandwich enzyme immunoassay technique that measures C1 in less than 4 hours. A polyclonal antibody specific for C1 has been pre-coated onto a microplate. C1 in standards and samples is sandwiched by the immobilized antibody and a biotinylated polyclonal antibody specific for C1, which is recognized by a streptavidin-peroxidase conjugate. All unbound material is then washed away and a peroxidase enzyme substrate is added. The color development is stopped and the intensity of the color is measured.