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- 详细信息
- 文献和实验
- 技术资料
- 库存:
大量
- 供应商:
AssayPro
- 检测范围:
0.04 μg/ml
- 检测方法:
ELISA
- 应用:
血清、血浆
- 样本:
25 μl
- 规格:
96 wells
α1抗胰蛋白酶α1-AT酶联免疫试剂盒
Introduction
Alpha-1 antitrypsin (A1AT) is a protein that protects the lungs. The liver usually makes the protein, and releases it into the bloodstream. A1AT is a major protease inhibitor that controls tissue degradation. A reduction of A1AT levels can cause a change in collagen metabolism (1). A1AT inhibits neutrophil elastase release into the lungs during inflammatory states (2). A1AT deficiency is an uncommon genetic disease (3) that can lead to emphysema (4), hepatitis, cirrhosis (5), and chronic obstructive pulmonary disease (COPD) (6).
Principal of the Assay
The AssayMax Human A1AT ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for detection of human A1AT in plasma and serum. This assay employs a quantitative competitive enzyme immunoassay technique that measures human A1AT in less than 3 hours. A polyclonal antibody specific for human A1AT has been pre-coated onto a 96-well microplate with removable strips. A1AT in standards and samples is competed with a biotinylated A1AT sandwiched by the immobilized antibody and streptavidin-peroxidase conjugate. All unbound material is then washed away and a peroxidase enzyme substrate is added. The color development is stopped and the intensity of the color is measured.
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文献和实验人α1 抗胰蛋白酶(α1-AT ) 酶联免疫分析( ELISA ) 试剂盒使用说明书 本试剂仅供研究使用 目的:本试剂盒用于测定人血清,血浆及相关液体样本中 α1 抗胰蛋白酶(α1-AT ) 含量。 实验原理: 本试剂盒应用双抗体夹心法测定标本中 人α 1 抗胰蛋白酶(α 1-AT ) 水平。用纯化的 人α 1 抗胰蛋白酶(α 1-AT ) 抗体包被微孔板,制成固相抗体,往包被单抗的微孔中依次加入 α
consists of a cooperation between monocytes and cytophilic antibodies able to bind to the Fc receptors present on the monocyte surface. Thus human IgG1 and IgG3 are the main isotypes effective in ADCI, whereas IgG2, IgG4, and IgM are ineffective
Assay (Invitrogen) as suggested by Illumina. Illumina Bead Array MicroRNA Detection Starting with 500 ng/sample of total RNA, mature microRNAs were amplified with the Illumina human v1 MicroRNA expression profiling kit containing primers for 743
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