Product Description:Affinity purified rabbit polyclonal anti-methylated lysine antibody is developed using a methylated protein as immunogen. The methyllysine-specific antibodies are affinity purified using Ne-monomethyllysine and dimehyllysine on Agarose as affinity matrix. The purified antibody was conjugated to fluorscein. The conjugated antibody was tested to localize the methylated proteins.
Species:Rabbit
Formulation:250 µg/mL in PBS, 50% glycerol.
Immunogen:Methylated KLH conjugates
Purification:N-e-monomethyllysine and -dimethyllysine on Agarose.
Specificity:Recognize proteins methylated on lysine residues (mono and di-methylysine). Not corss-react with acetylated proteins. Cross-reactivity to trimethyllysine is not tested.
Conjugation:with fluorescein isothiocyanate (FITC), FITC/Ab molar ratio: approximately 10:1
Applications:IF, 1:50-1:100
Storage & Stability:Store product at –20℃. One year from date of shipping.