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Blue Pippin全自动DNA脉冲场电泳回收仪
—整合脉冲场功能,全自动运行,既可以筛选回收小片段DNA,也可以实现对几十kb的大片段更加精准的回收
• Sage Science 公司
位于美国马萨诸塞州,2010年成立,专注于核酸领域的样品制备
全球超过3000家客户的认可,国内装机数量400台左右
2万篇文献中应用,包括众多Nature、 Science、 Cell系列的文章
DNA片段筛选回收领域的行业金标准
• Blue Pippin全自动DNA脉冲场电泳回收仪
采用双通道电泳回收专利技术,全自动运行
实现高精度回收、微量样品回收、高得率回收、大片段(几kb、几十kb甚至几百kb)回收、宽范围回收
实现对常规手工切胶和磁珠方法无法回收或回收不好的DNA样品筛选回收
技术原理
采用独家双通道电泳回收专利技术(Patents 8,361,298 and 8,361,299),搭配多模式回收选项,全程自动化运行
上样后,软件设定日标片段范,设备自动成分离、检测、回收全流程·通过荧光Marker精准计算片段迁移速度,自动控制回收时机,无需人工值守·回收完成后,直接用移液器吸取目标片段,无缝衔接下游实验
产品特点
精一精确度高(回收精确度达93-99%)
微一低起始量(低至ng级,控制时间回收,不直接检测样品)
高一高回收率(50-80%的回收得率)
大一大分子DNA (脉冲场电泳分离回收kb-几百kb的大片段DNA)
省一省时间(5分钟的手工操作时间,无需额外制胶和切胶)
应用方向
- NGS建库
- Nanopore、华大、齐碳、普译、今是等长片段文库建库
- 小RNA/small RNA文库构建
- Pacbio HiFi文库构建
- 超长测序(Ultra long Sequencing)文库构建
典型案例
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三代测序精准彻底的去除小片段,最大限度的发挥三代测序长度长的优势 Blue Pippin自带脉冲场电泳程序,对于10kb以上的大片段依然有较好的分离效果,甚至回收起点可以设置为40kb,用于超长测序(Ultra long Sequencing)建库
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代表客户

发表文献
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1.Sieriebriennikov, Bogdan, et al. "Transcriptional interferences ensure one olfactory receptor per ant neuron." Nature (2025): 1-9. 2.Lynch, Ryan C., et al. "Domesticated cannabinoid synthases amid a wild mosaic cannabis pangenome." Nature (2025): 1-10. 3.Morales, Ariadna E., et al. "Bat genomes illuminate adaptations to viral tolerance and disease resistance." Nature (2025): 449-458. 4.Feng, Jia-Wu, et al. "A haplotype-resolved pangenome of the barley wild relative Hordeum bulbosum." Nature (2025): 429-438. 5.Tsukahara, Sayuri, et al. "Centrophilic retrotransposon integration via CENH3 chromatin in Arabidopsis." Nature (2025): 744-748. 6.Avni, Raz, et al. "A pangenome and pantranscriptome of hexaploid oat." Nature (2025): 1-9. 7.Herklotz, V., et al. "Bimodal centromeres in pentaploid dogroses shed light on their unique meiosis." Nature (2025): 1-10. 8.Jiao, Chengzhi, et al. "Pan-genome bridges wheat structural variations with habitat and breeding." Nature (2025): 384-393. 9.Healey, Adam L., et al. "Newly identified sex chromosomes in the Sphagnum (peat moss) genome alter carbon sequestration and ecosystem dynamics." Nature Plants (2023): 1-17. 10.Munson-McGee, Jacob H., et al. "Decoupling of respiration rates and abundance in marine prokaryoplankton." Nature (2022): 1-7. |
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文献和实验|
1.Sieriebriennikov, Bogdan, et al. "Transcriptional interferences ensure one olfactory receptor per ant neuron." Nature (2025): 1-9. 2.Lynch, Ryan C., et al. "Domesticated cannabinoid synthases amid a wild mosaic cannabis pangenome." Nature (2025): 1-10. 3.Morales, Ariadna E., et al. "Bat genomes illuminate adaptations to viral tolerance and disease resistance." Nature (2025): 449-458. 4.Feng, Jia-Wu, et al. "A haplotype-resolved pangenome of the barley wild relative Hordeum bulbosum." Nature (2025): 429-438. 5.Tsukahara, Sayuri, et al. "Centrophilic retrotransposon integration via CENH3 chromatin in Arabidopsis." Nature (2025): 744-748. 6.Avni, Raz, et al. "A pangenome and pantranscriptome of hexaploid oat." Nature (2025): 1-9. 7.Herklotz, V., et al. "Bimodal centromeres in pentaploid dogroses shed light on their unique meiosis." Nature (2025): 1-10. 8.Jiao, Chengzhi, et al. "Pan-genome bridges wheat structural variations with habitat and breeding." Nature (2025): 384-393. 9.Healey, Adam L., et al. "Newly identified sex chromosomes in the Sphagnum (peat moss) genome alter carbon sequestration and ecosystem dynamics." Nature Plants (2023): 1-17. 10.Munson-McGee, Jacob H., et al. "Decoupling of respiration rates and abundance in marine prokaryoplankton." Nature (2022): 1-7. |
一般指青霉属(Penicillium)。为分布很广的半知菌纲中的一属,和曲霉属有亲缘关系,有二百几十种,代表种是灰绿青霉( Penicillium glaucum),从土壤或空气中很易分离,分枝成帚状的分生孢子从菌丝体伸向空中,各顶端的小梗产生链状的青绿—褐色的分生孢子。根据分生孢子顶端的膨大与否,与曲霉属( AsPergillus)相区别。其语源来自其形状(帚状, Penicillus)。子囊壳为封闭型。该属菌产生一种特殊物质。自从弗莱明( A. Fleming, 1929)发现特异
Celestine Blue 2.5g Ferric Ammonium Sulphate (Iron Alum) 25g Glycerin 70ml Distilled water 500ml Dissolve ferric ammonium sulphate in cold distilled water. Add celestine blue and boil for a few mins. Cool, filter and add glycerin
SECTIONS 4micron Paraffin wax sections SOLUTIONS Alcian Blue pH 3.1 Alcian Blue 1g 0.5% Acetic Acid 100ml Alcian Blue pH 2.5 Alcian Blue 1g
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