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- 详细信息
- 文献和实验
- 技术资料
- 英文名:
1-Biotinylamino-3,6,9-trioxaundecane-11-ol
- 库存:
1
- 供应商:
甄准生物
- 保存条件:
Hygroscopic, -20°C Freezer, Under Inert Atmosphere
- 规格:
25mg / 250mg
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文献和实验Acid-guanidinium thiocyanate-phenol-chloroform RNA purification
citrate, pH 7 (DEPC-treated, autoclaved) Sarcosyl (N-lauryl sarcosine, Sigma) b-mercaptoethanol (Sigma) 2M sodium acetate, pH4 (DEPC-treated, autoclaved) 3M sodium acetate, 100mM magnesium acetate, pH 5.2 Absolute ethanol Propan-2-ol
Pollen Development in Anthers of Arabidopsis
apart from the anthers. 10. Carefully remove excess Acetic Acid with a piece of filter paper and immediately add a drop of 1% Propionic Carmine to stain the chromosomes (see Hint #1). 11. Gently tap the preparation with a copper rod
DNA Extraction from Cheek Cells
buffer containing 1μl proteinase K (1mg/ml) and 1% SDS. 6.Incubate at 370 C overnight in a water bath. 7.Add 1ml SE buffer 8.Add 500μl 6M NaCl 9.Add 2.5ml chloroform 10.Mix for 60 min in an over-the-top rotator. 11.Vortex for 20 sec. 12
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