LD Biopharma Recombinant Human

Recombinant Human HLF-11R Protein，品牌LD Biopharma，货号HTF-1233，规格20 µg / Vial。 基因符号：HLF (TEL-2; TELB) 登录号：NP_002117 物种：Human 质量控制：1. Purity: > 90% by SDS-PAGE. 2. DNA binding activity: not tested yet. 制剂组成：0.2 mg/ml, sterile-filtered, in 20 mM pH 8.0 Tris-HCl Buffer, with proprietary formulation of NaCl, KCl, EDTA, arginine, DTT and Glycerol. 英文说明：Human Hepatic leukemia factor (HLF) gene encodes a member of the proline and acidic-rich (PAR) protein family, a subset of the bZIP transcription factors. The encoded protein forms homodimers or heterodimers with other PAR family members and binds sequence-specific promoter elements to activate transcription. Chromosomal translocations fusing portions of this gene with the E2A gene cause a subset of childhood B-lineage acute lymphoid leukemias. Alternatively spliced transcript variants have been described, but their biological validity has not been determined. Recent data also indicated that HLF plays a role in induction of multipotential hematopoietic progenitor from iPS cell via in vitro reprogramming. Full-length of human HLF cDNA (295aa, which derived from BC036093) was constructed with codon optimization and expressed with a small T7-His-TEV cleavage site Tag (31aa) fusion at its N-terminal and 11R tag at C-terminal. This protein was expressed in E. coli as inclusion bodies, refolded using our unique “temperature shift inclusion body refolding” technology and chromatographically purified. 应用信息：1. May be used for in vitro HLF mediated HSC differentiation regulation study using either 11R mediated or “ProFectin” based intracellular delivery of this protein. 2. May be used as specific protein substrate for kinase and ubiquitin (Sumo pathway) related enzyme functional screening assays. 3. May be used for HLF protein-protein interaction mapping. 4. As immunogen for specific antibody production. 本品仅供科研使用。实际构建、质量控制、保存及使用要求请以批次文件为准；网站目录不代表当前库存或实际供货承诺。