LD Biopharma Recombinant Human

Recombinant Human TFEB-11R Protein，品牌LD Biopharma，货号HTF-3208，规格40 µg / Vial。 基因符号：TFEB (ALPHATFEB; BHLHE35; TCFEB) 登录号：NP_009093 物种：Human 质量控制：Purity: > 91 % by SDS-PAGE. 制剂组成：0.4 mg/ml, sterile-filtered, in 20 mM pH 8.0 Tris-HCl Buffer, with proprietary formulation of NaCl, KCl, EDTA, Sucrose, DTT & Others. 英文说明：Recent studies suggest that a number of genes encoding lysosomal proteins exhibit coordinated transcriptional behavior and are regulated by the transcription factor EB (TFEB). As transcription factor, TFEB specifically recognizes and binds E-box sequences (5'-CANNTG-3'). Efficient DNA-binding requires dimerization with itself or with another MiT/TFE family member such as TFE3 or MITF. In association with TFE3, activates the expression of CD40L in T-cells, thereby playing a role in T-cell-dependent antibody responses in activated CD4+ T-cells and thymus-dependent humoral immunity. TFEB also specifically recognizes and binds the CLEAR-box sequence (5'-GTCACGTGAC-3') present in the regulatory region of many lysosomal genes, leading to activate their expression. It thereby plays a central role in expression of lysosomal genes. Acts as a positive regulator of autophagy by promoting expression of genes involved in autophagy. TFEB specifically recognizes the gamma-E3 box, a subset of E-boxes, present in the heavy-chain immunoglobulin enhancer. TFEB plays a role in the signal transduction processes required for normal vascularization of the placenta. Full-length human TFEB (475aa, Isoform-1. derived from BC032448) gene was constructed with codon optimized gene synthesis technology, and fusion with 28 aa N-terminal T7/His /TEV cleavage site Tag and 11 arginine (11R tag) at C-terminal. It was expressed in E.coli as inclusion bodies. The final product was refolded using our unique “temperature shift inclusion body refolding” technology and chromatographically purified. 应用信息：May be used for studying TFEB mediated autophagy–lysosome activation regulation for maintaining intracellular amino acid pools in vitro using intracellular protein delivery of recombinant TFEB-11R protein by adding protein directly into culture medium. May be used for mapping TFEB protein-protein interaction. May be used as specific substrate protein for kinase, and ubiquitin (Sumo pathway) related enzyme functional screening assays. Highly purified human TFEB antigen for specific antibody development. 本品仅供科研使用。实际构建、质量控制、保存及使用要求请以批次文件为准；网站目录不代表当前库存或实际供货承诺。