Black Paramagnetic Polyethylen

Methods for DNA isolation

or cosmid DNA of interest into a 12 X 75 mm Falcon tube containing 2 ml of LB media supplemented with the appropriate antibiotic (typically ampicillin at 100 ug/ml) and incubate at 37deg C 8-10 hours with shaking at 250 rpm. Transfer the culture

SOUTHERN BLOTS

10%SDS 5g Powdered Milk (Any, we use LowFat Nestle) 60g Polyethylene Glycol (6-8kd) 250ml 20xSSPE 100ml Formamide 70ml Salmon Sperm DNA 3mg/ml, sonicated in 15 ml aliquots, boiled

Methods for DNA isolation(DNA分离方法)

of bacteria harboring the plasmid or cosmid DNA of interest into a 12 X 75 mm Falcon tube containing 2 ml of LB media supplemented with the appropriate antibiotic (typically ampicillin at 100μg/ml)and incubate at 37deg C 8-10 hours with shaking at 250 rpm