Human IGF-I Quantikine SixPak

A Simple and Reliable Assay of Proteoglycan Synthesis by Cultured Chondrocytes

A simple and reliable method to measure proteoglycan synthesis by chondrocytes in culture is described. Confluent chondrocytes in 24-well plates are labeled for 24–72 h with 35 SO4 2− in the presence of stimulating agents. At the end

Endothelial Cell Systems - Instructions For Use

) 2.0 ml hFGF-B (human Fibroblast Growth Factor - Basic with heparin) 0.5 ml VEGF (Vascular Endothelial Growth Factor) 0.5 ml Ascrobic Acid (Vitamin C) 0.2 ml Hydrocortisone 0.5 ml Long R3-IGF-1 (Human Recombinant Insulin-like Growth Factor) 0.5 ml

Establishment of Stable Transfectant of CHO Lec Cells

expression vectors with strong promoter such as immediate early CMV (pcDNA3.1 etc.) or human EF-1a (pEF1/V5-His etc.). pBJ-5/GS vector also gives you high expression but requires additional cloning strategy. Materials Medium Sigma provides