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- Sigma-Aldrich
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- Y0001143
- 2026年09月10日
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- 文献和实验
- 技术资料
- 保存条件：
试剂
- 保质期：
试剂
- 英文名：
-
- 库存：
有
- 供应商：
福州木辰
- CAS号：
78628-80-5
- 规格：
Y0001143
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文献和实验of fatty acid synthesis inhibitors (refs. 7,8). Materials Reagents DTT was from Fisher (BP172-5); beta-mercaptoethanol was from Bio-Rad (161-0710). [2-14C]Malonyl-CoA (60 mCi/mmol, Perkin Elmer, NEC612), ACP (Sigma, A7303) was pretreated with 3 mM DTT
Gel-elongation assay for type II fatty acid synthesis
Elmer, NEC612 ), ACP (Sigma, A7303 ) was pretreated with 3 mM DTT on ice for 20 min, aliquoted and stored at -80 °C. Cerulenin and thiolactomycin were purchased from Sigma. Triclosan could be obtained from VWR, 003384. Urea, PVDF membrane, 10X Tris
Gel-elongation assay for type II fatty acid s
]Malonyl-CoA (60 mCi/mmol, Perkin Elmer, NEC612), ACP (Sigma, A7303) was pretreated with 3 mM DTT on ice for 20 min, aliquoted and stored at -80 °C. Cerulenin and thiolactomycin were purchased from Sigma. Triclosan could be obtained from VWR, 003384. Urea
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