艾博抗Abcam 山羊抗小鼠IgG1重链 货号:ab9165

Isotype-specific immunostaining with mouse primary antibodies on mouse tissue

) Briefly, substitute your anti H+L pan-reactive secondary antibody with an anti-isotype heavy chain specific secondary. Be aware of possible odd crossreactivties (e.g. many goat anti mouse IgG 1 heavy chain do stain rat IgG 2a

Rapid Isotyping of Mouse Monoclonal Antibodies

for 5% of mouse antibodies) has limited practical value. In contrast, the heavy-chain isotype is very informative (see Table 1 ); protein A or protein G binding, euglobulin properties, complement binding, kinetics of digestion with papain or pepsin, clearance

Immunoglobulin Class Switching: In Vitro Induction and Analysis

of the gene segments coding for the constant region of the Ig heavy chain, although the Ig heavy chain variable region is retained. This process changes the effector functions of the corresponding antibody (Ab). Much of our current understanding