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- 文献和实验
- 技术资料
- 抗体名:
MDM2 (Phospho-Tyr394) Antibody 抗体
- 抗体英文名:
MDM2 (Phospho-Tyr394) Antibody
- 靶点:
MDM2
- 应用范围:
WB
- 宿主:
Rabbit
- 适应物种:
Human, Mouse
- 保质期:
12 months from date of receipt.
- 抗原来源:
详询
- 目录编号:
orb1414851
- 级别:
科研
- 库存:
99
- 供应商:
Biorbyt
- 标记物:
Unconjugated
- 克隆性:
Polyclonal
- 保存条件:
详询
- 免疫原:
详询
- 规格:
100 ul
描述: Rabbit polyclonal antibody to MDM2 (Phospho-Tyr394).
别名: E3 ubiquitin-protein ligase Mdm2 (EC 6.3.2.-) (Double minute 2 protein) (Hdm2) (Oncoprotein Mdm2) (p53-binding protein Mdm2)
应用稀释比例: WB: WB 1:500-2000, ELISA 1:10000-20000
研究领域: Cell Biology
UniProt ID: Q00987
保存条件: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
备注: For research use only.

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文献和实验Flow Cytometric Analysis of MDM2-Mediated Growth Arrest
synthesis or repair can be detected in partially denatured DNA with a BrdU-specific fluorescent antibody. Subsequent staining of transfected MDM2 with a different fluorochrome provides information about whether transfected cells make significant progression
运用Cell Based Elisa检测信号通路蛋白和磷酸化蛋白
using Phospho-p38 antibody (A) and Total-p38 antibody (B). Cell Based Elisa were performed directly in the 96-well plates using Phospho-p38 and Total-p38 antibodies (C). Figure 3: JNK detection with FACECells were serum-starved for 16 hours
。所以,我们建议使用微波炉或高压锅加热煮沸以达到最佳抗原修复效果。抗体:Phospho–Stat3 (Tyr705)(D3A7) XP. Rabbit mAb #9145样本:石蜡包埋人类肺部肿瘤样本煮沸设备:浴锅 (左),微波炉 (中),高压锅 (右)免疫染色—封闭在 IHC–P 中,我们建议在室温下在含有 Tween20 的 TBST 缓冲液和 5% 正常山羊血清 (NGS) 中封闭样品,时长 1 小时,以防止非特异性背景染色。在进行 IHC–F 时,在含有 0.3% Triton. X–100 的 1X
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