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- 文献和实验
- 技术资料
- 保存条件:
2-8度
- 保质期:
1年
- 英文名:
DUT
- 库存:
999
- 供应商:
上海淳麦生物
- 规格:
/5ug/20ug/1mg
Recombinant Human Deoxyuridine Triphosphatase
英文简称:
DUT
中文名称:
重组人脱氧尿苷三磷酸酶
背景资料:
Deoxyuridine Triphosphatase(DUT) is a ubiquitous enzyme that functions in nucleotide metabolism. Deoxyuridine Triphosphatase, in the presence of magnesium ions, is responsible for hydrolyzing dUTP to dUMP and diphosphate. This reaction is imperative for kDUT Human Recombinant fused with a 21 amino acid His tag at N-terminus produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 204 amino acids(70-252 a.a.) and having a molecular mass of 21.6kDa. The DUT is purified by proprietary chromatographic techniques.ping the intracellular dUTP concentration low so that uracil does not become incorporated into DNA. Extensive integration of uracil into DNA can eventually lead to cell death. This suests that DUT is crucial for cell viability, further implying that it is a CMBIOtive target for anticancer therapy.
产品描述:
DUT Human Recombinant fused with a 21 amino acid His tag at N-terminus produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 204 amino acids(70-252 a.a.) and having a molecular mass of 21.6kDa. The DUT is purified by proprietary chromatographic techniques.
来源宿主:
大肠杆菌中重组表达
溶解建议:
提供的重组人脱氧尿苷三磷酸酶产品形式为无菌的无色溶液.
保存建议:
人脱氧尿苷三磷酸酶蛋白产品可整瓶4℃保存2-4周,长期保存请置于-20℃.推荐添加载体蛋白(0.1% HSA或BSA)以长期保存,请避免反复冻融.
应用领域:
重组人脱氧尿苷三磷酸酶产品可广泛应用于酶活性研究、代谢研究、功能研究、蛋白互作研究、ELISA检测、Western Blot、免疫组化等生物医学研究领域。
关键词:
重组人脱氧尿苷三磷酸酶、Recombinant Human Deoxyuridine Triphosphatase、DUT、重组蛋白、多肽、酶类、原核表达、His标签
其他简介:
CMBIO




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文献和实验Site-directed Mutagenesis using Dut-Ung system-Molecular
Hello, I have been trying over the last few months to introduce a single point mutation in a looped portion of an RNA, using the dut-ung method of mutagenesis. So far, I have had little success. I have a feeling that this problem
In vitro Mutagenesis with dut ung single stranded DNA
In vitro Mutagenesis with dut ung single stranded DNA Steve Hahn last modified 3/3/00 Kinase Oligonucleotide: (gel purified oligonucleotides give highest mutation frequency) 0.5 microgram oligonucleotide
A Protocol for Site-Directed Mutagenesis Employing a Uracil-Containing Phagemid Template
two key enzymes, dUTPase (dut) and uracil N-glycosylase (ung) , will synthesize DNA with a small number of uracil bases substituted for thymine. Uracil containing single-stranded DNA (ssDNA) prepared from this dut − , ung − host strain can be used
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