重组人血管内皮生长因子受体-1 D1-7(FLT1 D7)

产品英文名称：
Recombinant Human Vascular Endothelial Growth Factor receptor-1 D1-7

英文简称:
FLT1 D7

中文名称:
重组人血管内皮生长因子受体-1 D1-7

背景资料:
Endothelial cells express thrSoluble FLT1 Human Recombinant fused with the Fc part of human IgG1 produced in baculovirus is disulfide-linked homodimeric， glycosylated， polypeptide containing 751 amino acids and having a molecular mass of 130 kDa. The soluble receptor protein contains only the first 7 extracellular domains （Met1-Thr751）， which contain all the information necessary for high a昆虫细胞中重组表达inity ligand binding. The FLT1 fc/Chimera is purified by proprietary chromatographic techniques. di昆虫细胞中重组表达erent vascular endothelial growth factor （VEGF） receptors， belonging to the family of receptor tyrosine kinases （RTKs）. They are named VEGFR-1 （Flt-1）， VEGFR-2 （KDR/Flk-1）， and VEGFR-3 （Flt-4）. Their expression is almost exclusively restricted to endothelial cells， but VEGFR-1 can also be found on monocytes. All VEGF-receptors have seven immunoglobulin-like extracellular domains， a single transmembrane region and an intracellular split tyrosine kinase domain. VEGFR-2 has a lower a昆虫细胞中重组表达inity for VEGF than the Flt-1 receptor， but a higher signalling activity. Mitogenic activity in endothelial cells is mainly mediated by VEGFR-2 leading to their proliferation. Di昆虫细胞中重组表达erential splicing of the flt-1 gene leads to the formation of a secreted， soluble variant of VEGFR-1 （sVEGFR-1）. No naturally occurring， secreted forms of VEGFR-2 have so far bSoluble FLT1 Human Recombinant fused with the Fc part of human IgG1 produced in baculovirus is disulfide-linked homodimeric， glycosylated， polypeptide containing 751 amino acids and having a molecular mass of 130 kDa. The soluble receptor protein contains only the first 7 extracellular domains （Met1-Thr751）， which contain all the information necessary for high a昆虫细胞中重组表达inity ligand binding. The FLT1 fc/Chimera is purified by proprietary chromatographic techniques.n reported. The binding of VEGF165 to VEGFR-2 is dependent on heparin.

产品描述:
Soluble FLT1 Human Recombinant fused with the Fc part of human IgG1 produced in baculovirus is disulfide-linked homodimeric， glycosylated， polypeptide containing 751 amino acids and having a molecular mass of 130 kDa. The soluble receptor protein contains only the first 7 extracellular domains （Met1-Thr751）， which contain all the information necessary for high a昆虫细胞中重组表达inity ligand binding. The FLT1 fc/Chimera is purified by proprietary chromatographic techniques.
来源宿主:
昆虫细胞中重组表达

溶解建议:
提供的人血管内皮生长因子受体-1 D1-7（FLT1 D7）产品为过滤灭菌的白色冻干粉末；该冻干粉末是从含1mg/ml人血管内皮生长因子受体-1 D1-7（FLT1 D7）的溶液（不含添加剂）中过滤灭菌并冻干产生的.我们建议加PBS以配制工作储存液到不低于50ug/ml，后继实验过程中可根据实验要求进一步稀释.

保存建议：
人血管内皮生长因子受体-1 D1-7（FLT1 D7）冻干粉末在室温可保存3周，或置于-18℃冻存用于长期保存.重新溶解后可以在4℃冷藏保存至少2-7天以上；如果想延长保存时间，建议分装（加载体蛋白如0.1% HSA或 BSA）后置于-18℃冻存.请避免反复冻融.
应用领域：
重组人血管内皮生长因子受体-1 D1-7产品可广泛应用于细胞增殖与分化研究、发育生物学研究、信号传导研究、药物靶点研究、功能研究、蛋白互作研究、ELISA检测、Western Blot、免疫组化等生物医学研究领域。

关键词：
重组人血管内皮生长因子受体-1 D1-7、Recombinant Human Vascular Endothelial Growth Factor receptor-1 D1-7、FLT1 D7、重组蛋白、多肽、生长因子、受体蛋白、昆虫细胞表达

其他简介：

 
CMBIO