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- 文献和实验
- 技术资料
- 保存条件:
2-8度
- 保质期:
1年
- 英文名:
SUMO2 GST
- 库存:
999
- 供应商:
上海淳麦生物
- 规格:
/5ug/20ug/1mg
Recombinant Human Small Ubiquitin-Related Modifier 2, GST
英文简称:
SUMO2 GST
中文名称:
重组人小泛素相关修饰物2,GST标签
背景资料:
SUMO2 is a member of the SUMO (small ubiquitin-like modifier) protein family. SUMO2 also known as SUMO-3 is a novel ubiquitin-like protein that can be conjugated to other proteins in a manner analogous to ubiquitination. It is a 95 amino acid peptide that is 46% identical and 66% homologous to sentrin-1. Sentrin interacts with the death domains of Fas, TNFR1, PML, Rad51 and Rad52 which are involved in repairing dsDNA breaks, and with GAP1. SUMO2 function is similar to that of ubiquitin by being bound to target proteins as part of a post-translational modification system. SUMO2 is involved in a variety of cellular processes, for example nuclear transport, transcriptional regulation, apoptosis, and protein stability as opposed to ubiquitin which targets proteins for degradation. SUMO2 is not active until the last two amino acids of the carboxy-terminus have bSUMO2 Human Recombinant full length protein expressed in E.coli, shows a 36 kDa band on SDS-PAGE(including GST tag). The SUMO2 is purified by proprietary chromatographic techniques.n cleaved o大肠杆菌中重组表达.
产品描述:
SUMO2 Human Recombinant full length protein expressed in E.coli, shows a 36 kDa band on SDS-PAGE(including GST tag). The SUMO2 is purified by proprietary chromatographic techniques.
来源宿主:
大肠杆菌中重组表达
溶解建议:
Sterile Filtered clear solution. SUMO2 at 100ug/ml in 50mM Tris-Acetate, pH7.5, 1mM EDTA and 20% Glycerol.
保存建议:
Store vial at -20℃ to -80℃. When stored at the recommended temperature, this protein is stable for 12 months. Please prevent freeze-thaw cycles.
应用领域:
重组人小泛素相关修饰物2,GST标签产品可广泛应用于功能研究、蛋白互作研究、ELISA检测、Western Blot、免疫组化等生物医学研究领域。
关键词:
重组人小泛素相关修饰物2,GST标签、Recombinant Human Small Ubiquitin-Related Modifier 2, GST、SUMO2 GST、重组蛋白、多肽、原核表达、标签蛋白、CMBIO
其他简介:
CMBIO




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文献和实验,只要互作蛋白间具有相互结合的结构域基础即可实验证明;Co-IP 实验是细胞内高表达目的蛋白,并通过 IgG 球珠-抗体-蛋白复合物体系,分离出互作的蛋白复合物,是细胞内的体内反应结合。2. GST pull-down 实验优点① 可验证蛋白质-蛋白质之间的直接互作。② GSH 谷胱甘肽偶联球珠亲和力强,洗脱纯度高。3. GST pull-down 实验缺点① 验证互作是在试管中进行的生化反应,不能够完全反应细胞内蛋白真实互作状态。② 融合表达的 GST 标签,肽链较长,可能会改变原目的蛋白的原有的折叠结构。
mL PBS 1X 4- Centrifuge (1pulse at 500g) 5- Resuspend in 10 mL PBS 1X (stable for one month at 4 _ C) b) Extract preparation: 1- Transform BL21 or any appropriate bacterial strain with your GST-fused cDNA. Always use freshly transformed cells
-PAGE 检测。 16) 收集含有带着GST标签的蛋白经 PBS 在 4 °C透析至少 1 hr,3次。 17) 液氮速冻并保存于 -80 °C。 配方 1. 2xYT 培养基 细菌蛋白胨 16g 酵母提取物 5g NaCl 5g H2O 800 ml 调整 pH=7.2 加 H2O到 1L. 高压灭菌 2. PBS 缓冲液 (pH=7.4) (1 L)
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