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- 文献和实验
- 技术资料
- 抗体名:
NGF Antibody 抗体
- 抗体英文名:
NGF Antibody
- 靶点:
NGF
- 浓度:
batch dependent
- 应用范围:
ELISA, NeA, WB
- 宿主:
Mouse
- 适应物种:
Human
- 保质期:
12 months from date of receipt.
- 抗原来源:
详询
- 目录编号:
orb1272665
- 级别:
科研
- 库存:
99
- 供应商:
Biorbyt
- 标记物:
Unconjugated
- 克隆性:
Monoclonal
- 保存条件:
详询
- 形态:
Lyophilized
- 免疫原:
详询
- 规格:
0.5 mg
产品子类型: Primary Antibody
描述: NGF Antibody
克隆号: M85-2B
别名: Nerve growth factor, NGFB, HSAN5, Beta-NGF, MGC161426, MGC161428 , Beta-nerve growth factorBeta-NGF
应用说明: Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of hB-NGF (10.0 ng/mL), a concentration of 2.7 - 4.0 μg/mL of this antibody is required. ELISA:In a sandwich ELISA (assuming 100 μL/well), a concentration of 2.0-4.0 μg/mL of this antibody will detect at least 200 pg/mL of recombinant human β-NGF when used with our biotinylated antigen affinity purified anti-human b-NGF as the detection antibody at a concentration of approximately 0.5-1.0 μg/mL.Western Blot:To detect hβ-NGF by Western Blot analysis this antibody can be used at a concentration of 4.0-8.0 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hβ-NGF is 4.0-8.0 ng/lane, under reducing or non-reducing conditions.
免疫原: beta-NGF antibody is produced in mice using highly pure (>98%) recombinant human beta-NGF as the immunizing antigen.
纯化方法: This IgG2bK antibody was purified from cell culture by Protein G affinity chromatography.
研究领域: Neuroscience
UniProt ID: P01138
NCBI 参考序列: P01138
保存条件: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
备注: For research use only.


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文献和实验Enzyme-Linked ImmunoSorbent Assay (ELISA) for NGF
A. DAY 1 PROCEDURE• ABSORPTION OF THE POLYCLONAL AND PREIMMUNE SERUMThe Goat Anti-Mouse (GAM) NGF polyclonal antibody recognizes 2.5B NGF and the Goat IgG (GIG) preimmune serum serves as a control for the antibody (Hazleton). The relative signal
Chimerization of a Monoclonal Antibody for Treating Hodgkin's Lymphoma
Ber-H2 which recognizes the human CD30 molecule with high specificity and affinity (2 ). CD30, a member of the nerve growth factor (NGF) tumor necrosis factor receptor (TNFR) superfamily (3 ), is strongly expressed on the pathognomonic Hodgkin (H
上海西唐生物科技有限公司 021-55229872, 65333639 www.westang.com 人神经生长因子 ( NGF )ELISA 试剂盒 ( 用于血清、血浆、细胞培养上清液和其它生物体液内 ) 原理 本实验采用双抗体夹心 ABC-ELISA 法。用抗人 NGF 单抗包被于酶标板上,标准品和样品中的 NGF与单抗结合,加入生物素化的抗人 NGF ,形成免疫复合
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