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- 文献和实验
- 技术资料
- 抗体名:
Metallothionein Antibody (HRP) 抗体
- 抗体英文名:
Metallothionein Antibody (HRP)
- 靶点:
Metallothionein
- 浓度:
1 mg/ml
- 应用范围:
WB
- 宿主:
Rabbit
- 适应物种:
Bacteria
- 保质期:
12 months from date of receipt.
- 抗原来源:
详询
- 目录编号:
orb55589
- 级别:
科研
- 库存:
99
- 供应商:
Biorbyt
- 标记物:
HRP
- 克隆性:
Polyclonal
- 保存条件:
详询
- 免疫原:
详询
- 规格:
100 ug
描述: Rabbit polyclonal antibody against Metallothionein-1 Conjugated to HRP
别名: Metallothionein, Metallothionein 1A, Metallothionein 1S, Metallothionein IA, Metallothionein II, Metallothionein-1A, Metallothionein-IA, Metallothionein2, Metallothionein 2, Metallothionein 2A, MT1, MT1A, MT1S, MT2, MT2A, MT 1A, MT IA, MT II, MT-1A, MT-IA, MT1A_HUMAN, MGC32848, MTC, pmtA, Probable Metallothionein, CES 1, CES1
应用稀释比例: WB (1:1000)
应用说明: A 1:1000 dilution was sufficient for detection of Metallothionein in 15 µg of pseudomonas aeruginosa purified Metallothionein (pmtA-GST) protein by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
免疫原: Synthetic peptide from the mid-protein of Pseudomonas aeruginosa Metallothionein
分子量: 36 kDa
防腐剂: 73.64mM Carbonate, 54.55mM Ethanolamine, 45.45mM Cyanoborohydride, 18.18mM Sodium Hydroxide, 0.23mM Citrate
纯化方法: Peptide Affinity Purified
特异性: Detects ~36 kDa.
研究领域: Epigenetics & Chromatin, Signal Transduction
UniProt ID: Q9I1X5
保存条件: Conjugated antibodies should be stored according to the product label
备注: For research use only.

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文献和实验Conjugation of Antibody to HRP
of Maleimide:HRP Weigh out 1.067 mg HRP for each mg of thiolated antibody. Add 1.1 mg Sulfo-SMCC per mg of HRP Add PBS to a final concentration of 8 mg/ml HRP Mix for 2 hours on a rotator. Desalt the HRP on PBS equilibrated column. Collect
Conjugation of Antibody to HRP
Conjugation of Antibody to HRP Author: Nanci Donacki Source: Contributed by Nanci Donacki Reagents
Monoclonal Antibody Production Protocol
- PBS + 0.1% Tween 20 or TBS + 0.1% Tween 20. - Blocking solution = 2% BSA (type V ) in PBS. (Add 0.02% azide for longer storage.) - Elisa buffer = 2% BSA + 0.1% Tween 20 in PBS ( azide optional ). - Enzyme linked antibody = Horseradish peroxidase
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