Metallothionein Antibody (HRP) 抗体,orb55589,Biorbyt产品图

Metallothionein Antibody (HRP)

抗体,orb55589,Biorbyt
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  • ¥7488
  • Biorbyt已认证
  • orb55589
  • 英国
  • 2026年07月24日
  • WB
  • Rabbit
  • Bacteria
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 抗体名:

      Metallothionein Antibody (HRP) 抗体

    • 抗体英文名:

      Metallothionein Antibody (HRP)

    • 靶点:

      Metallothionein

    • 浓度:

      1 mg/ml

    • 应用范围:

      WB

    • 宿主:

      Rabbit

    • 适应物种:

      Bacteria

    • 保质期:

      12 months from date of receipt.

    • 抗原来源:

      详询

    • 目录编号:

      orb55589

    • 级别:

      科研

    • 库存:

      99

    • 供应商:

      Biorbyt

    • 标记物:

      HRP

    • 克隆性:

      Polyclonal

    • 保存条件:

      详询

    • 免疫原:

      详询

    • 规格:

      100 ug

    类别: Antibodies

    描述: Rabbit polyclonal antibody against Metallothionein-1 Conjugated to HRP

    别名: Metallothionein, Metallothionein 1A, Metallothionein 1S, Metallothionein IA, Metallothionein II, Metallothionein-1A, Metallothionein-IA, Metallothionein2, Metallothionein 2, Metallothionein 2A, MT1, MT1A, MT1S, MT2, MT2A, MT 1A, MT IA, MT II, MT-1A, MT-IA, MT1A_HUMAN, MGC32848, MTC, pmtA, Probable Metallothionein, CES 1, CES1

    应用稀释比例: WB (1:1000)

    应用说明: A 1:1000 dilution was sufficient for detection of Metallothionein in 15 µg of pseudomonas aeruginosa purified Metallothionein (pmtA-GST) protein by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.

    免疫原: Synthetic peptide from the mid-protein of Pseudomonas aeruginosa Metallothionein

    分子量: 36 kDa

    防腐剂: 73.64mM Carbonate, 54.55mM Ethanolamine, 45.45mM Cyanoborohydride, 18.18mM Sodium Hydroxide, 0.23mM Citrate

    纯化方法: Peptide Affinity Purified

    特异性: Detects ~36 kDa.

    研究领域: Epigenetics & Chromatin, Signal Transduction

    UniProt ID: Q9I1X5

    保存条件: Conjugated antibodies should be stored according to the product label

    备注: For research use only.

    产品细节图片1
    Western blot analysis of Pseudomonas aeruginosa Purified protein showing detection of ~36 kDa (9.07 kDa Metallothionein and 26.4 kDa GST) Metallothionein protein using Rabbit Anti-Metallothionein Polyclonal Antibody. Lane 1: Molecular Weight Ladder (MW). Lane 2: Blank. Lane 3: Pseudomonas aeruginosa purified protein with GST tag. Load: 25 μg. Block: 1% milk, 2% BSA. Primary Antibody: Rabbit Anti-Metallothionein Polyclonal Antibody at 1:1000 for 16 hours at 4°C. Secondary Antibody: Mouse Anti-Rabbit IgG: AP at 1:1000 for 60 min at RT. Color Development: Alkaline phosphate activity colorimetric assay. Predicted/Observed Size: ~36 kDa (9.07 kDa Metallothionein and 26.4 kDa GST).

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    图标文献和实验
    相关实验
    • Conjugation of Antibody to HRP

      of Maleimide:HRP Weigh out 1.067 mg HRP for each mg of thiolated antibody. Add 1.1 mg Sulfo-SMCC per mg of HRP Add PBS to a final concentration of 8 mg/ml HRP Mix for 2 hours on a rotator.  Desalt the HRP on PBS equilibrated column.  Collect

    • Conjugation of Antibody to HRP

        Conjugation of Antibody to HRP Author: Nanci Donacki Source: Contributed by Nanci Donacki     Reagents

    • Monoclonal Antibody Production Protocol

      - PBS + 0.1% Tween 20 or TBS + 0.1% Tween 20. - Blocking solution = 2% BSA (type V ) in PBS. (Add 0.02% azide for longer storage.) - Elisa buffer = 2% BSA + 0.1% Tween 20 in PBS ( azide optional ). - Enzyme linked antibody = Horseradish peroxidase

    图标技术资料

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