- ￥7605
- Biorbyt
- orb535759
- 英国
- 2026年07月29日
- IHC, WB
- Rabbit
- Human, Mouse, Rat
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- 文献和实验
- 技术资料
- 抗体名：
MDM2 Antibody (Biotin) 抗体
- 抗体英文名：
MDM2 Antibody (Biotin)
- 靶点：
MDM2
- 浓度：
1 mg/ml
- 应用范围：
IHC, WB
- 宿主：
Rabbit
- 适应物种：
Human, Mouse, Rat
- 保质期：
12 months from date of receipt.
- 抗原来源：
详询
- 目录编号：
orb535759
- 级别：
科研
- 库存：
99
- 供应商：
Biorbyt
- 标记物：
Biotin
- 克隆性：
Polyclonal
- 保存条件：
详询
- 免疫原：
详询
- 规格：
100 ug
描述: Rabbit polyclonal antibody against MDM2 conjugated to Biotin
别名: MDM2, Double minute 2 protein, Hdm2, MTBP, Mouse Double Minute 2, HDMX, ACTFS, p53 Binding Protein Mdm2, Ubiquitin protein ligase E3 Mdm2, Mdm2 p53 E3 ubiquitin protein ligase homolog, MDM2BP, p53-binding protein Mdm2, Oncoprotein Mdm2, Hdm 2, E3 ubiquitin-protein ligase Mdm2, MDM 2, EC:6.3.2.-, MDM2_HUMAN, MDM2 oncogene E3 ubiquitin protein ligase, Murine Double Minute Chromosome 2, Mdm2 transformed 3T3 cell double minute 2 p53 binding protein (mouse) binding protein 104kDa
应用稀释比例: WB (1:1000); IHC (1:50)
免疫原: Synthetic peptide from the mid-protein of Human MDM2 (aa. 100-200)
分子量: 50 kDa
防腐剂: 136.36mM Ethanolamine, 133.23 mM Chlorides, 9.55mM Phosphates, 9.55mM Sodium Bicarbonate.
纯化方法: Peptide Affinity Purified
特异性: Detects ~50 kDa. ~50 kDa band is isoform 2 or 3.
研究领域: Cell Biology
UniProt ID: Q00987
NCBI 参考序列: NP_001138811.1
Entrez 基因 ID: 4193
保存条件: Conjugated antibodies should be stored according to the product label
备注: For research use only.
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文献和实验The Avidin-Biotin Complex (ABC) Method and Other Avidin-Biotin Binding Methods
These methods involve, at their core, the vitamin biotin and the protein avidin, which bind together irreversibly. By establishing a biotin link, through avidin, between the horseradish peroxidase enzyme and a secondary antibody reagent
Flow Cytometric Analysis of MDM2-Mediated Growth Arrest
synthesis or repair can be detected in partially denatured DNA with a BrdU-specific fluorescent antibody. Subsequent staining of transfected MDM2 with a different fluorochrome provides information about whether transfected cells make significant progression
The Avidin-Biotin Complex(ABC) Method
, between the horseradish peroxidase enzyme and a secondary antibody reagent, enzyme localization can be achieved at the site of primary antibody interaction with the specimen (see ) Fig. 1 ). The biotin molecule is small and can be easily conjugated to immunoglobulin
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