HSP70 (P. falciparum) Antibody

类别: Antibodies

描述: Rabbit polyclonal to Hsp70 (PerCP). Hsp70 genes encode abundant heat-inducible 70-kDa hsps (hsp70s). In most eukaryotes hsp70 genes exist as part of a multigene family. They are found in most cellular compartments of eukaryotes including nuclei, mitochondria, chloroplasts, the endoplasmic reticulum and the cytosol, as well as in bacteria. The genes show a high degree of conservation, having at least 5O% identity. The N-terminal two thirds of hsp70s are more conserved than the C-terminal third. Hsp70 binds ATP with high affinity and possesses a weak ATPase activity which can be stimulated by binding to unfolded proteins and synthetic peptides. When hsc70 (constitutively expressed) present in mammalian cells was truncated, ATP binding activity was found to reside in an N-terminal fragment of 44 kDa which lacked peptide binding capacity. Polypeptide binding ability therefore resided within the C-terminal half. The structure of this ATP binding domain displays multiple features of nucleotide binding proteins. All hsp70s, regardless of location, bind proteins, particularly unfolded ones. The molecular chaperones of the hsp70 family recognize and bind to nascent polypeptide chains as well as partially folded intermediates of proteins preventing their aggregation and misfolding. The binding of ATP triggers a critical conformational change leading to the release of the bound substrate protein. The universal ability of hsp70s to undergo cycles of binding to and release from hydrophobic stretches of partially unfolded proteins determines their role in a great variety of vital intracellular functions such as protein synthesis, protein folding and oligomerization and protein transport. PfHsp70-I (PF08_0054) is the major cytosolic Hsp70 in Plasmodium falciparum. It is abundantly expressed in the blood stages of the parasite and is thought to constitute 1-2% of total parasite protein. It is induced upon heat shock. It is present in the parasite in different complexes with PfHsp90 and some PfHsp40 ...

别名: HSP70, Heat shock 70 kDa protein, 74.3 kDa protein, Cytoplasmic antigen, HSP70_PLAFA, PF3D7_0818900

应用稀释比例: WB (1:2000), ICC/IF (1:50)

应用说明: 0.15 µg/ml was sufficient for detection of PfHSP70 in 20 µg of P. falciparum lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.

免疫原: His-tagged and purified PfHSP70, C-terminus (AA 365-681)

分子量: 70kDa

防腐剂: 95.46mM Phosphate, 2.48mM MES and 2mM EDTA

纯化方法: Protein A Purified

特异性: Detects ~ 70kDa. Specific to P. falciparum and does not cross-react to any protein from Human erythrocytes.

研究领域: Cancer Biology, Cell Biology, Protein Biochemistry, Signal Transduction

UniProt ID: P11144

NCBI 参考序列: M19753

保存条件: Conjugated antibodies should be stored according to the product label

备注: For research use only.