TNF-R1 Antibody (Biotin) 抗体，orb151862，Biorbyt产品图

TNF-R1 Antibody (Biotin) 抗体，or

b151862，Biorbyt
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  • ￥7371
  • Biorbyt已认证
  • orb151862
  • 英国
  • 2026年07月29日
  • ICC, IF, IHC, IP, WB
  • Rabbit
  • Bovine, Canine, Human, Monkey, Mouse, Rabbit, Rat
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武汉博欧特生物科技有限公司
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    • 技术资料
    • 抗体名：

      TNF-R1 Antibody (Biotin) 抗体

    • 抗体英文名：

      TNF-R1 Antibody (Biotin)

    • 靶点：

      TNF-R1

    • 浓度：

      1 mg/ml

    • 应用范围：

      ICC, IF, IHC, IP, WB

    • 宿主：

      Rabbit

    • 适应物种：

      Bovine, Canine, Human, Monkey, Mouse, Rabbit, Rat

    • 保质期：

      12 months from date of receipt.

    • 抗原来源：

      详询

    • 目录编号：

      orb151862

    • 级别：

      科研

    • 库存：

      99

    • 供应商：

      Biorbyt

    • 标记物：

      Biotin

    • 克隆性：

      Polyclonal

    • 保存条件：

      详询

    • 免疫原：

      详询

    • 规格：

      100 ug

    类别: Antibodies

    描述: Rabbit polyclonal antibody against TNFR1 conjugated to Biotin

    别名: Tumor necrosis factor receptor 1, TNFR-1, TNFRSF1A, TNFAR, TNFR1

    应用稀释比例: WB (1:1000), IHC (1:100), ICC/IF (1:100)

    应用说明: 1 µg/ml was sufficient for detection of TNFR1 in 20 µg of Hela lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.

    免疫原: Peptide corresponding to AA 20-43 of the mouse TNF-R1 sequence, identical to rat and human over those residues

    分子量: 55kDa

    防腐剂: 136.36mM Ethanolamine, 133.23 mM Chlorides, 9.55mM Phosphates, 9.55mM Sodium Bicarbonate.

    纯化方法: Peptide Affinity Purified

    特异性: Detects ~55kDa. Other bands present may be the result of oligomerization, self-aggregation and/or cleavage of the TNF-R1 extracellular domain.

    研究领域: Cell Biology

    UniProt ID: P19438

    NCBI 参考序列: P19438

    Entrez 基因 ID: 8666

    保存条件: Conjugated antibodies should be stored according to the product label

    备注: For research use only.

    产品细节图片1
    Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-TNF-R1 Polyclonal Antibody. Tissue: Cervical cancer cell line (HeLa). Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-TNF-R1 Polyclonal Antibody at 1:100 for 12 hours at 4°C. Secondary Antibody: APC Goat Anti-Rabbit (red) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Golgi apparatus membrane. Magnification: 100x. (A) DAPI (blue) nuclear stain. (B) Anti-TNF-R1 Antibody. (C) Composite.

    产品细节图片2
    Immunohistochemistry analysis using Rabbit Anti-TNF-R1 Polyclonal Antibody. Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative Solution. Primary Antibody: Rabbit Anti-TNF-R1 Polyclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1:50 for 1 hour at RT. Localization: dermis.

    产品细节图片3
    Western blot analysis of Mouse Liver cell lysates showing detection of ~55 kDa TNF-R1 protein using Rabbit Anti-TNF-R1 Polyclonal Antibody. Lane 1: Molecular Weight Ladder (MW). Lane 2: Mouse Liver cell lysates. Load: 15 μg. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-TNF-R1 Polyclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~55 kDa.

    产品细节图片4
    Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-TNF-R1 Polyclonal Antibody. Tissue: Cervical cancer cell line (HeLa). Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-TNF-R1 Polyclonal Antibody at 1:100 for 12 hours at 4°C. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Golgi apparatus membrane. Magnification: 20x. (A) DAPI (blue) nuclear stain. (B) Anti-TNF-R1 Antibody. (C) Composite.

    产品细节图片5
    Western blot analysis of Human A549 showing detection of ~ 50 kDa TNF-R1 protein using Rabbit Anti-TNF-R1 Polyclonal Antibody. Lane 1: MW Ladder, Lane 2: A549. Load: 30 ug. Block: 5% BSA in TBST. Primary Antibody: Rabbit Anti-TNF-R1 Polyclonal Antibody at 1:1000 for 2 hours at RT with shaking. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:4000 for 1 hour at RT with shaking. Color Development: Chemiluminescent for HRP (Moss) for 5 min in RT. Predicted/Observed Size: ~ 50 kDa. Other Band (s): ~90-100kDa. Other bands can be explained by a few factors, such as oligomerization, self-aggregation, cleavage of the TNFR1 extracellular domain, etc.

    Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-TNF-R1 Polyclonal Antibody. Tissue: HaCaT cells. Species: Human. Fixation: Cold 100% methanol at -20°C for 10 minutes. Primary Antibody: Rabbit Anti-TNF-R1 Polyclonal Antibody at 1:100 for 12 hours at 4°C. Secondary Antibody: FITC Goat Anti-Rabbit at 1:50 for 1-2 hours at RT in dark. Localization: Punctate nuclear staining, dotty staining in cytoplasm.

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