NG2 Recombinant Rabbit Monoclonal Antibody 抗体,orb704325,Biorbyt产品图

NG2 Recombinant Rabbit Monoclo

nal Antibody 抗体,orb704325,Biorbyt
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  • ¥2223 - 5265
  • Biorbyt已认证
  • orb704325
  • 英国
  • 2026年07月22日
  • WB
  • Rabbit
  • Human
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 抗体名:

      NG2 Recombinant Rabbit Monoclonal Antibody 抗体

    • 抗体英文名:

      NG2 Recombinant Rabbit Monoclonal Antibody

    • 靶点:

      CSPG4

    • 浓度:

      1mg/ml

    • 应用范围:

      WB

    • 宿主:

      Rabbit

    • 适应物种:

      Human

    • 保质期:

      12 months from date of receipt.

    • 抗原来源:

      详询

    • 目录编号:

      orb704325

    • 级别:

      科研

    • 库存:

      99

    • 供应商:

      Biorbyt

    • 标记物:

      Unconjugated

    • 克隆性:

      Recombinant

    • 保存条件:

      详询

    • 形态:

      Liquid

    • 亚型:

      IgG

    • 免疫原:

      详询

    • 规格:

      25 ul/50 ul/100 ul

    规格:25 ul产品价格:¥2223.0
    规格:50 ul产品价格:¥3744.0
    规格:100 ul产品价格:¥5265.0
    类别: Antibodies

    产品子类型: Primary Antibody

    描述: NG2 Recombinant Rabbit Monoclonal Antibody

    克隆号: B5C0

    别名: CSPG4A; HMW-MAA; MCSP; MCSPG; MEL-CSPG; MSK16; NG2; 4732461B14Rik; AN2; CSPG4_HUMAN; CSPG4; Chondroitin sulfate proteoglycan NG2; Melanoma chondroitin sulfate proteoglycan; Melanoma-associated chondroitin sulfate proteoglycan; CSPG4_MOUSE; Proteoglycan AN2; Kiaa4232; chondroitin sulfate proteoglycan 4; chondroitin sulfate proteoglycan 4 (melanoma-associated)

    应用稀释比例: WB=1:500-2000

    免疫原: A synthesized peptide derived from human CSPG4 (2250-2322/2322aa)

    分子量: 280 kDa

    预测反应性: Mouse, Rat

    防腐剂: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.

    纯化方法: Affinity purified by Protein A

    研究领域: Cancer Research, Cardiovascula, Neurogenesis, Neuroscience, Oligodendrocyte Marker, Tumor Biology

    UniProt ID: Q6UVK1

    保存条件: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

    备注: For research use only.

    产品细节图片1
    Blank control: SHG-44. Primary Antibody (green line): Rabbit Anti-NG2 antibody, dilution: 1:50, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody: Goat anti-rabbit IgG-AF488, dilution: 1:1000. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

    产品细节图片2
    Fixative-fixed, paraffin embedded (mouse brain tissue), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (NG2) Monoclonal Antibody, Unconjugated (orb704325) at 1:50 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

    产品细节图片3
    Fixative-fixed, paraffin embedded (rat brain tissue), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (NG2) Monoclonal Antibody, Unconjugated (orb704325) at 1:50 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

    产品细节图片4
    Sample: Lane 1: SiHa cell lysates, Primary: Anti-NG2 (orb704325) at 1:500 dilution, Secondary: Goat Anti-Rabbit IgG - HRP at 1:5000 dilution, Predicted band size: 248 kD, Observed band size: 280 kD.

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