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- 文献和实验
- 技术资料
- 供应商:
广州市左克生物
- 适应物种:
Human
- 规格:
96T
ProductInformation
KE00291isasolidphasesandwichEnzymeLinked-Immuno-SorbentAssay(SandwichELISA).ThehumanLIGHT/TNFSF14ELISAkitistobeusedtodetectandquantifyproteinlevelsofendogenoushumanLIGHT/TNFSF14.TheassayrecognizeshumanLIGHT/TNFSF14.AnantibodyspecificforhumanLIGHT/TNFSF14hasbeenpre-coatedontothemicrowells.ThehumanLIGHT/TNFSF14proteininsamplesiscapturedbythecoatedantibodyafterincubation.Followingextensivewashing,anotherantibodyofbiotinylatedspecificforhumanLIGHT/TNFSF14isaddedtodetectthecapturedhumanLIGHT/TNFSF14protein.Forsignaldevelopment,Streptavidin-HRPisadded,followedbyTetramethyl-benzidine(TMB)reagent.Solutioncontainingsulfuricacidisusedtostopcolordevelopmentandthecolorintensitywhichisproportionaltothequantityofboundproteinismeasurableat450nmwiththecorrectionwavelengthsetat630nm.
Productname HumanLIGHT/TNFSF14ELISAKit Tests 1X96wellplate Sampletype Serum,Plasma,Cellculturesupernatant,Saliva备注:对未验证样本,我们提供技术支持和售后保障。 Assaytype Sandwich Sensitivity 7.0pg/mL Range 62.5-4000pg/mL Reactivity Human Testedapplications SandwichELISA GeneID(NCBI) 8740
属性:
Properties
StorageInstructions Allthereagentsarestoredat2-8℃for6monthsor-20℃for12months.Refertotheprotocolforfurtherstorageinstructions. Synonyms HVEML,CD258,HVEML,LIGHT,LTg,TNFSF14,TR2引用文献数:
常见问题解答:
FAQ
1、我的样本稀释液有限,如何处理稀释倍数较大的样本?我们建议您对样本进行梯度稀释。例如,人血清样本需要1000000倍稀释时,您可以分3个梯度,每个梯度100倍,进行稀释:
第一步:取2uL人血清样本,加入198uL样本稀释液,混匀得到100倍稀释的人血清样本
第二步:取2uL100倍稀释的人血清样本+198uL样本稀释液进行稀释,混匀得到10000倍稀释的人血清样本
第三步:取2uL10000倍稀释的人血清样本+198uL样本稀释液进行稀释,混匀得到1000000倍稀释的人血清样本。
如果样本需要进一步稀释,您可以使用更多梯度。除了我们推荐的样本稀释液,不建议您使用其他溶液或者水稀释。如果您的样本稀释液仍然不足,请联系我们的客服为您提供更多样本稀释液。

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文献和实验volume of 35 ml at room temperature. 2) Add the diluted buffy coat on top of 15 ml of Lymphoprep. 3) Centrifuge at 160 x g for 20 minutes at 20°C. Allow to decellerate without brakes. 4) Remove 20 ml of supernatant
RAT/MOUSE GROWTH HORMONE ELISA KIT
实验原理 This assay is a Sandwich ELISA based, sequentially, on: 1) capture of rat or mouse Growth Hormone molecules from samples to the wells of a microtiter plate coated by a pre-titered amount of anti-Growth Hormone
as its pH will increase and reduce yields. 2) When adequate separation of bands has occurred, carefully excise the DNA fragment of interest using a UV light box ensuring that as much agarose gel as possible has been removed. Avoid more than 30
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