Matrices listed below were spiked with certain level of TNFSF14/LIGHT and the recovery rates were calculated by comparing the measured value to the expected amount of TNFSF14/LIGHT in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
86-99
93
EDTA plasma(n=5)
85-102
95
heparin plasma(n=5)
92-104
96
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of TNFSF14/LIGHT and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.