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文献和实验cDNA synthesis using superscript II
SuperScript II 1 µl water 4 µl Prepare this mix as a master mix and add then 20 µl to each reaction. Synthesize the cDNA in a thermocycler: 1.
E.coli Total RNA Labeling Protocol for Spotted Microarray
Note: Start with 20 mg of total RNA for each labeling reaction. All solutions that can be filtered should be filtered. Cy dyes are light sensitive and should ALWAYS be handled in dim light. RNA Preparation If RNA is in ethanol, spin down 20 mg
Modified Total RNA Protocol for Heart, Muscle, Brain and Skin tissue
-through liquid and reuse 50 ml tube in step 8. 8. Add 15 ml RNA Wash Buffer II, cap the 50 ml collecting tube and centrifuge for 5 min at 3,500-5,000 x g at room temperature. Again, discard flow-through liquid and reuse 50 ml tube in step 9. 9. Repeat
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