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- 文献和实验
- 技术资料
- 保存条件:
Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4oC); After reconstitution under sterile conditions for 3 months (-70oC).
- 英文名:
Recombinant Mouse Tslp, His-tagged
- 库存:
1000
- 规格:
100mg
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文献和实验High-Throughput Purification of PolyHis-Tagged Recombinant Fusion Proteins
Methods for the efficient overexpression and purification of recombinant proteins are of paramount importance for biotechnology. In particular, for the era of functional genomics that we have entered after sequencing complete
Recombinant DNA Engineering, Or Cloning Genes In Plasmids
tagged (with GFP or HA in these examples) protein of interest.Insertion of DNA of interest in a plasmid Pure DNA of interest is obtained by PCR or from another plasmid and is inserted in the vector plasmid by ligation or recombination or even PCR
proteins.It is a convenient alternative to sonication for extracting recombinant proteins from the cytoplasm of Escherichia coli cells carrying expression vectors,especially when many proteins are to be processed in parallel. In this article I’ll summarize
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