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文献和实验that you can download from the Invitrogen website. For the Two colour FISH, we recommend to use the Alexa Fluor 488 dye (FISH Tag™ DNA Green Kit, Cat. No. F32947) and the Alexa Fluor 555 dye (FISH Tag™ DNA Orange Kit, Cat. No. F32948). Note that for the Nick Translation
, all amine-reactive dyes. This kit provides two types of specially modified polystyrene microspheres, the ArC™ reactive beads (Component A) that bind any of the amine-reactive dyes, and the ArC™ negative beads (Component B), that have no reactivity. Using
DETECTION OF ß-GALACTOSIDASE AND ALKALINE PHOSPHATASE ACTIVITIES IN TISSUE
). Several substrates yield colored or fluorescent soluble products which are useful when quantifying ß-gal activity (McCaman and Robins, 1959, Miller 1972) or visualizing transduced cells live in vivo (Krasnow et al. 1991, Nirenberg and Cepko, 1993, Lin et al. 1994
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