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文献和实验). Mix well and incubate at room temp. for 5 min. Spin down the silica particles (glass milk), 5 sec., remove supernatant. (The DNA in the solution will now hopefully be bound to the silica particles). Wash the silica pellet with 800 μl wash solution
A quick method to isolate plant
.for 5 min. Spin down the silica particles (glass milk),5 sec.,remove supernatant.(The DNA in the solution will now hopefully be bound to the silica particles). Wash the silica pellet with 800μl wash solution (from the GeneClean II kit). Repeat the wash
A quick method to isolate plant genomic DNA.
well. Keep the solution on ice for i 30 min, (precipitation of proteins). Spin down the precipitations at 7000 rpm, 5 min. Transfer 300 ul of the supernatant to a new eppendorf tube. Add 900 ul NaI (GeneClean II), + 20 ul "glass milk" (silica particles
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