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文献和实验Chemistry of Minor Groove Binder–Oligonucleotide Conjugates
Figure 8.4.4 UV melting curves of DNA duplexes formed from distamycin‐type MB‐ODNs and their complements. First derivative plots of helix‐coil transitions at 260 nm for 3′‐MB‐ODN duplexes are shown. Distamycin
fluorescence when in solution, but emits a strong fluorescent signal upon binding to double-stranded DNA. TaqMan Probes TaqMan probes depend on the 5'- nuclease activity of the DNA polymerase used for PCR to hydrolyze an oligonucleotide that is hybridized
Synthesis and Application of Highly Reactive Amino Linkers for Functional Oligonucleotides
by using the hydrophobic interaction of the monomethoxytrityl group with a reverse?phase resin. Second, the introduction of the carbamate linkage reduces the pK a value of the neighboring primary amine, resulting in an increase in the conjugation yields
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