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- 文献和实验
- 技术资料
- 免疫原:
inquire
- 亚型:
Human IgG1 - kappa
- 形态:
Powder
- 保存条件:
Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized antibodies are shipped at ambient temperature.
- 克隆性:
Monoclonal
- 标记物:
Unconjugated
- 适应物种:
Human
- 级别:
Research use only
- 库存:
In Stock
- 供应商:
缔码生物(武汉)科技有限公司
- 宿主:
N/A
- 应用范围:
ELISA,Flow Cyt
- 浓度:
详见COA
- 靶点:
4-1BB Ligand
- 抗体英文名:
Anti-4-1BB Ligand(SC113.153 biosimilar) mAb
- 抗体名:
Anti-4-1BB Ligand(SC113.153 biosimilar) mAb
- 规格:
50μg
| 产品类型 | Biosimilar reference antibodies |
|---|---|
| 靶点 | 4-1BB Ligand |
| Uniprot ID | P41273 |
| 背景 | Research grade biosimilar. Not for use in therapeutic or diagnostic procedures for humans or animals. |
| 通用名 | SC113.153 |
| 货期 | In Stock |
| 产品英文名称 | Anti-4-1BB Ligand(SC113.153 biosimilar) mAb |
| 内毒素 | Less than 1.0 EU/μg by the LAL method. For <1 EU/mg requirements, please contact us for customization. |
| 保质期 | 12 months from date of dispatch |
| 无菌性 | Products are supplied non-sterile. For cell culture applications, dilute in appropriate medium and sterile-filter (0.22 µm) prior to use. |
| 储存与运输 | Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized antibodies are shipped at ambient temperature. |
| 别名 | TNFSF9, TNFL9 |
| 应用 | ELISA,Flow Cyt |
| 有无偶联 | Unconjugated |
| 组分 | Lyophilized from sterile PBS, pH 7.4. Normally 5 % – 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution. |
| 宿主种属 | N/A |
| IgG亚型 | Human IgG1 - kappa |
| 反应种属 | Human |
| 推荐稀释比 | ELISA 1:5000-10000,Flow Cyt 1:100 |
| 应用范围 | Research use only |

Figure 1. ELISA plate pre-coated by 2 μg/mL (100 μL/well) Human 4-1BB Ligand Protein, mFc-His Tag (PME100054) can bind Anti-4-1BB Ligand(SC113.153 biosimilar) mAb (BME100300) in a linear range of 1.6-8.0 ng/mL.

Figure 2. ELISA plate pre-coated by 2 μg/mL (100 μL/well) Anti-4-1BB Ligand(SC113.153 biosimilar) mAb (BME100300) can bind Biotinylated Human 4-1BB Ligand Protein, mFc-His Tag (PME100054B) in a linear range of 40–200 ng/mL. In order to specifically detect PME100054B, HRP Conjugated-Streptavidir was used as detection antibody.

Figure 3. Flow cytometry analysis with 0.5 μg/ml Anti-4-1BB Ligand(SC113.153 biosimilar) mAb (BME100300) on HEK293 cells transfected with Human 4-1BB Ligand protein (Blue histogram) and HEK293 transfected with irrelevant protein (Red histogram) .
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文献和实验原指对络合物(如蛋白质)中心原子配位的原子或原子团,但通常是指与蛋白质进行特异结合的低分子物质,例如除与酶进行特异结合的底物、辅酶、调节因子外,也指与存在于细胞膜上的各种受体蛋白质分子进行特异结合的外源凝集素、抗原、抗体、激素和神经传递物质等。
【讨论】抗体和那些不能说的"秘密"-抗体选择的PASS标准 (请加精)
purification)的方法来纯化多克隆抗体,该技术的 关键在于使用的亲和原(affinity ligand)。 为了得到效价更高,特异性更好的抗体, 一流的抗体公司尽可能使用全蛋白做为亲和原去得到优质的抗体,而且常常还会进行第二 步的蛋白A或G的分化。相反,使用多肽作为亲和原,纯化到的多抗表位有限,多抗的效价 和特异性都会大打折扣。 因此, 一流的抗体公司的多克隆抗体的效价,特异性的优势明显,而且很多多克隆抗体可 以用于中和和阻断实验。
人可溶性CD137 (4-1BB)ELISA 试剂盒 ( 用于血清、血浆、细胞培养上清液和其它生物体液内 ) 原理 本实验采用双抗体夹心 ABC-ELISA 法。用抗人 CD137/4-1BB/TNFRSF9 单抗包被于酶标板上,标准品和样品中的 4-1BB与单抗结合,加入生物素化的抗人 4-1BB ,形成免疫复合物连接在板上,辣根过氧化物酶标记的 Streptavidin 与生物素结合,加入
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