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- 文献和实验
- 技术资料
- 免疫原:
inquire
- 亚型:
Mouse IgG2a - Kappa
- 形态:
Powder
- 保存条件:
Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.
- 克隆性:
Monoclonal
- 标记物:
Unconjugated
- 适应物种:
Human
- 级别:
Research use only
- 库存:
In Stock
- 供应商:
缔码生物(武汉)科技有限公司
- 宿主:
Mus musculus
- 应用范围:
ELISA, Flow Cyt
- 浓度:
详见COA
- 靶点:
CD19
- 抗体英文名:
Anti-CD19(FMC63 biosimilar) mAb
- 抗体名:
Anti-CD19(FMC63 biosimilar) mAb
- 规格:
50μg
| 产品类型 | Biosimilar reference antibodies |
|---|---|
| 靶点 | CD19 |
| Uniprot ID | P15391 |
| 背景 | Research grade biosimilar. Not for use in therapeutic or diagnostic procedures for humans or animals. |
| 通用名 | FMC63 |
| 货期 | In Stock |
| 产品英文名称 | Anti-CD19(FMC63 biosimilar) mAb |
| 内毒素 | Less than 1.0 EU/μg by the LAL method. For <1 EU/mg requirements, please contact us for customization. |
| 保质期 | 12 months from date of dispatch |
| 无菌性 | Products are supplied non-sterile. For cell culture applications, dilute in appropriate medium and sterile-filter (0.22 µm) prior to use. |
| 储存与运输 | Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature. |
| 别名 | CD19;4;VID3;GC12802 |
| 应用 | ELISA, Flow Cyt |
| 有无偶联 | Unconjugated |
| 组分 | Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution. |
| 宿主种属 | Mus musculus |
| IgG亚型 | Mouse IgG2a - Kappa |
| 反应种属 | Human |
| 推荐稀释比 | ELISA 1:5000-10000, Flow Cyt 1:100 |
| 应用范围 | Research use only |

Figure 1. Flow cytometry analysis with 1 μg/mL Anti-CD19 mAb (BME100094) on HEK293 cells transfected with Human CD19 protein (Blue histogram) or HEK293 transfected with irrelevant protein (Red histogram).

Figure 2. ELISA plate pre-coated by 2 μg/mL (100 μL/well) Human CD19 (M75I,L82V,F83L) Protein, hFc Tag (PME101557) can bind Anti-CD19(FMC63 biosimilar) mAb (BME100094) in a linear range of 3.20–400 ng/mL.

Figure 3. ELISA plate pre-coated by 2 μg/mL (100 μL/well) Human CD19 (M75V,R76S,F85S) Protein, hFc Tag (PME101558) can bind Anti-CD19(FMC63 biosimilar) mAb (BME100094) in a linear range of 3.20–400 ng/mL.
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文献和实验CD19 分子 CD19 常用单克隆抗体或代号: B4,Leu12 主要表达细胞: B,Pre,FDC [B] 分子质量(kDa)和结构: gp90(IgSF) 功 能: 与CD21、CD81组成复合物,调节B细胞活化 CD19 Response regulator that plays a dominant role in establishing signaling
脾细胞与小鼠骨髓瘤细胞(P3-X63/Ag8)融合,融合的细胞既获得了亲代脾细胞分泌特异性抗体的特性,又具有骨髓瘤细胞大量繁殖的能力,成为一种既能分泌特异性抗体又能长命的杂交瘤细胞。该技术为抗体的分子生物学研究提供了全新的手段。极大地促进了免疫学,遗传学,分子生物学的快速发展。 但传统抗体目前也面临诸多问题,如: 每使用一管新抗体都需要进行滴定测试; 多种同型对照,实验设计繁琐; 需要添加Fc阻断试剂; 抗体不佳,阳性细胞群不明显; 抗体作为细胞生物学和生物化学中最重要的试剂之一,科学界每年在这类
CD63 分子 CD63 常用单克隆抗体或代号: RUU- SP2.28,CLB 主要表达细胞: Pt,M,Mac,(G,T,B) [Pt] 分子质量(kDa)和结构: gp53,血小板颗粒蛋白(TM4- SF) 功 能: 血小板活化,中性粒细胞- 活化内皮细胞粘附 CD63 Aka lysosomal-membrane-associated glycoprotein 3, LAMP
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