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- 文献和实验
- 技术资料
- 免疫原:
inquire
- 亚型:
Human IgG1 - kappa
- 形态:
Powder
- 保存条件:
Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.
- 克隆性:
Monoclonal
- 标记物:
Unconjugated
- 适应物种:
Human
- 级别:
Research use only
- 库存:
In Stock
- 供应商:
缔码生物(武汉)科技有限公司
- 宿主:
Humanized
- 应用范围:
ELISA
- 浓度:
详见COA
- 靶点:
IL21
- 抗体英文名:
Anti-IL21(avizakimab biosimilar) mAb
- 抗体名:
Anti-IL21(avizakimab biosimilar) mAb
- 规格:
50μg
| 产品类型 | Biosimilar reference antibodies |
|---|---|
| 靶点 | IL21 |
| Uniprot ID | Q9HBE4 |
| 背景 | Research grade biosimilar. Not for use in therapeutic or diagnostic procedures for humans or animals. |
| 通用名 | BOS161721 |
| 货期 | In Stock |
| 产品英文名称 | Anti-IL21(avizakimab biosimilar) mAb |
| 内毒素 | Less than 1.0 EU/μg by the LAL method. For <1 EU/mg requirements, please contact us for customization. |
| 保质期 | 12 months from date of dispatch |
| 无菌性 | Products are supplied non-sterile. For cell culture applications, dilute in appropriate medium and sterile-filter (0.22 µm) prior to use. |
| 储存与运输 | Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature. |
| 别名 | CVID11;Za11 |
| 应用 | ELISA |
| 有无偶联 | Unconjugated |
| 组分 | Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution. |
| 宿主种属 | Humanized |
| IgG亚型 | Human IgG1 - kappa |
| 反应种属 | Human |
| 推荐稀释比 | ELISA 1:5000-10000 |
| 应用范围 | Research use only |

Figure 1. ELISA plate pre-coated by 2 μg/mL (100 μL/well) Human IL21 Protein, hFc Tag (PME101088) can bind Anti-IL21 Neutralizing antibody (BME100103) in a linear range of 2.44–156.25 ng/mL. In order to specifically detect BME100103, mouse anti-human Fab-specific antibody was used as detection antibody.

Figure 2. ELISA plate pre-coated by 2 μg/mL (100 μL/well) Human IL21 Protein, His Tag (PME100556) can bind Anti-IL21(avizakimab biosimilar) mAb (BME100103) in a linear range of 3.2–80 ng/mL.
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文献和实验ERLKSLIQKM IHQHLS2, FeaturesDL-IL21-Hu人白介素 21 (IL21) 酶联免疫吸附检测试剂盒预期应用本试剂盒运用双抗体夹心 ELISA 法定量测定人血清、血浆、组织匀浆、细胞裂解液、细胞培养上清液和其他生物液体中 IL21 含量。标本的采集与保存1. 血清:将收集于血清分离管的全血标本在室温放置 2 小时或 4℃ 过夜,然后 1000×g 离心 20 分钟,取上清即可,或将上清置于-20℃ 或-80℃ 保存,但应避免反复冻融。2. 血浆:用 EDTA 或肝素作为抗凝剂采集
基础。 传统的NK细胞扩增技术是在NK细胞培养体系中添加IL2、IL15等细胞因子以促进NK细胞增殖,但由于扩增效率不高且纯度不够未能得到广泛应用。近年来,有人采用基因工程的方法在K562细胞上同时表达IL-15、CD137L 等分子, 并以此细胞来刺激NK细胞的增殖。IL-15和CD137L联合刺激是PBMC 中NK细胞特异性扩增的基础:作为膜蛋白,通过细胞间的接触刺激,用以诱导NK细胞扩增。IL15能够促进NK细胞的分化成熟,而IL21能够联合IL15分子促进NK细胞的分化成熟,所以本研究在稳定
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