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- CAS号：
738-70-5
- 规格：
500mg
Product Introduction
Bioactivity
| 名称 | Trimethoprim |
| 描述 | Trimethoprim (NSC-106568) is a Dihydrofolate Reductase Inhibitor Antibacterial. The mechanism of action of trimethoprim is as a Dihydrofolate Reductase Inhibitor, and Cytochrome P450 2C8 Inhibitor, and Organic Cation Transporter 2 Inhibitor. |
| 体内活性 | Trimethoprim通过干扰细菌二氢叶酸还原酶的作用,抑制四氢叶酸的合成。抑制作用使得细菌DNA复制所必需的核苷酸缺乏,在某些情况下导致胸腺嘧啶死亡引起的细胞死亡。 |
| 存储条件 | Powder: -20°C for 3 years | In solvent: -80°C for 1 year Shipping with blue ice/Shipping at ambient temperature. |
| 溶解度 | 10% DMSO+40% PEG300+5% Tween 80+45% Saline : 4 mg/mL (13.78 mM), Sonication is recommended. DMSO : 122 mg/mL (420.23 mM), Sonication is recommended. Ethanol : < 1 mg/mL (insoluble or slightly soluble) |
| 关键字 | urinary tract infections | Trimethoprim | Thymidylate synthase | tetrahydrofolate | Shigellosis | RNASynthesis | RNA Synthesis | reductase | protein | Pneumocystis pneumonia | NSC106568 | NSC 106568 | NIH-204 | NIH204 | metabolism | Inhibitor | inhibit | Influenza Virus | Hsps | folate | DNASynthesis | DNA Synthesis | dihydrofolate | DHFR | Bacterial | Antimicrobial | Antifolate | Antibiotic | aggregation |
| 相关产品 | Guanidine hydrochloride | Levulinic acid | Doxycycline | Neomycin sulfate | Terbinafine hydrochloride | Dimethyl sulfoxide | Sodium bicarbonate | Hyaluronic acid sodium (MW 20 kDa) | Thymidine | Sodium diacetate | Sulfamethoxazole sodium | D(+)-Raffinose pentahydrate |
| 相关库 | Inhibitor Library | Bioactive Compound Library | Bioactive Compounds Library Max | Failed Clinical Trials Compound Library | Anti-Aging Compound Library | Bitter Compound library | FDA-Approved Drug Library | Anti-Viral Compound Library | Immunology/Inflammation Compound Library | Drug Repurposing Compound Library | Anti-Cancer Clinical Compound Library | Anti-Cancer Drug Library |
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文献和实验细胞培养技术中，细胞计数是一项基本功，对于标准化培养条件以及需要精确定量的实验来说都非常关键。这里介绍使用血细胞计数器对细胞进行计数的经典方法以及中间一些需要注意的细节。制备细胞悬液：对于贴壁生长的细胞，我们需要首先使用胰酶消化的方法使细胞从培养皿表面脱落根据需要加入合适体积的培养基，将细胞进行中和及稀释，以得到均质的细胞悬液。要求尽可能将细胞吹打散开，不要残留任何细胞团准备血细胞计数器：使用70%乙醇将盖玻片和血细胞计数器清洁干净将将盖玻片润湿（使用水或呼一口气，目的是使盖玻片与血细胞
miRNA，pri- miRNA)。 2) Pri-miRNA在Drosha-DGCR8复合体的作用下形成长度约60-70个核苷酸的发夹状RNA，成为前体miRNA(precursor miRNA，pre-miRNA)。 3) 随后，pre-miRNA在Exprotin-5复合物[2]的作用下被转运出胞核，在胞浆中由Dicer剪切成为miRNA(microRNA)复合体，miRNA(microRNA)复合物(RNA-induced silencing comlex，RISC)与该miRNA
James Hardwick CNBr Cleavage Procedure
tube containing 200 microliters of 100mg/ml CNBr in 70% formic acid. CNBr stocks are 200 milligrams/ml in 70% formic acid and are stored at -70°C. 70% formic acid is made from 98-100% formic acid and MQH2O. Optional: If you want to know
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