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- CAS号：
147591-46-6
- 规格：
1mg/1mLx10mM(inDMSO)/5mg/10mg/25mg/50mg/100mg/200mg
| 规格： | 1mg | 产品价格： | ￥138.0 |
|---|---|---|---|
| 规格： | 1mLx10mM(inDMSO) | 产品价格： | ￥306.0 |
| 规格： | 5mg | 产品价格： | ￥278.0 |
| 规格： | 10mg | 产品价格： | ￥430.0 |
| 规格： | 25mg | 产品价格： | ￥774.0 |
| 规格： | 50mg | 产品价格： | ￥1224.0 |
| 规格： | 100mg | 产品价格： | ￥1960.0 |
| 规格： | 200mg | 产品价格： | ￥2896.0 |
Product Introduction
Bioactivity
| 名称 | ID-8 |
| 描述 | ID-8, a DYRK inhibitor, sustains embryonic stem cell self-renewal in long-term culture. |
| 细胞实验 | Briefly, the hESCs in feeder-free culture are dissociated completely with 0.05% trypsin-EDTA and seeded at 104 cells per well in Matrigel-coated 6-well culture plates and cultured in MEF-CM. Various concentrations of Wnt3, IQ-1, ID-8, and/or ICG-001 are supplemented into the culture media at the onset of seeding and then continuously until the end of culturing. For all assays, the cell and colony morphology are examined under a microscope, and the replating efficiency is examined by counting the number of colonies after 7 days of culture. (Only for Reference) |
| 激酶实验 | In Vitro Enzymatic Assay for Histone Deacetylases: In vitro activities of the 11 recombinant human zinc-dependent HDAC enzymes are detected by ?uorigenic release of 7-amino-4-methylcoumarin from substrate upon deacetylase enzymatic activity. A series of dilutions of the unique HDAC6 compound, tubacin, and SAHA are prepared with 10% DMSO in HDAC assay buffer, and 5 μL of the dilution was added to a 50-μL reaction so that the ?nal concentration of DMSO is 1% in all of the reactions. The enzymatic reactions are conducted in duplicate at 37 °C for 30 min in a 50-μL mixture containing HDAC assay buffer, 5 μg BSA, an HDAC substrate, an HDAC enzyme, and a test compound. After enzymatic reactions, 50 μL of 2× HDAC developer is added to each well, and the plate is incubated at room temperature for an additional 15 min. Fluorescence intensity is measured at an excitation of 360 nm and an emission of 460 nm using a Synergy microplate reader. Negative (no enzyme, no inhibitor, a drug with no HDAC inhibition activity) and positive controls (known HDAC inhibitor SAHA) are included in the assays. IC50 is determined at the drug concentration that results in 50% reduction of HDAC activity compared with the control. |
| 体内活性 | ID-8通过Sox2-Oct3 / 4激活维持Nanog基因表达,能够可逆地维持ESC细胞的长期培养。ID-8在Wnt存在的情况下,调节Wnt /β-连环蛋白信号,增加CBP /β-连环蛋白,来维持hESC的未分化状态,并维持细胞的增殖和存活。 |
| 存储条件 | Powder: -20°C for 3 years | In solvent: -80°C for 1 year Shipping with blue ice/Shipping at ambient temperature. |
| 溶解度 | DMSO : 29.8 mg/mL (99.9 mM), Sonication is recommended. |
| 关键字 | tyrosine | stem | regulated | phosphorylation | kinase | Inhibitor | inhibit | ID-8 | ID 8 | ESC | embryonic | DYRK | dual-specificity | Dual specificity tyrosine regulated kinase | Dual specificity tyrosine phosphorylation regulated kinase | cell |
| 相关产品 | Harmine hydrochloride | MBM-55S | 2-Amino-4-hydroxypyrrolo[2,3- d]pyrimidi | ML 315 | EHT 1610 | CK2/ERK8-IN-1 | AnnH31 | LDN-192960 | ARN25068 | YK-2-69 | GNF4877 | RD0392 |
| 相关库 | Inhibitor Library | Bioactive Compound Library | Anti-Cancer Compound Library | Bioactive Compounds Library Max | Cell Cycle Compound Library | Kinase Inhibitor Library | NO PAINS Compound Library | Stem Cell Differentiation Compound Library | Membrane Protein-targeted Compound Library | Tyrosine Kinase Inhibitor Library | Post-Translational Modification Compound Library |
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网络 第十九章 杂环化合物和生物碱 杂环化合物在自然界分布很广，其数量几乎占已知有机化合物的三分之一，用途也很多。许多重要的物质如叶绿素、血红素、核酸以及临床应用的一些有显著疗效的天然药物和合成药物等，都含有杂环化合物的结构。生物碱多是中草药的有效成分，绝大多数是含氮的杂环化合物。本章内容与医学关系密切，具有重要意义。
分子中具有高能键的化合物。在生物体内主要有上式（Ⅰ）和（Ⅱ）两种结构的高能化合物。其作用为贮藏能量（例如 ATP、肌酸磷酸）和作为产生 ATP源的中间代谢产物（例如磷酸烯醇丙酮酸）以及合成反应的中间物质（例如酰基辅酶 A），在蛋白质的结构（ conformation）变化周期的第一阶段上（例如钠钾 ATP酶的羧基磷酸中间体）也具有重要作用。多数高等化合物具有磷酸基，但也有例外。
由不同种元素组成的纯净物叫做化合物。化合物一般有固定的组成。化合物的组成一般可用化学式表示。化合物具有确定的物理性质和化学性质，不同于其组成元素的性质。化合物中的元素不能用简单的机械方法或物理方法分开，而必须用化学方法才能分离。
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化合物 ID-8【147591-46-6】
￥138 - 2896
