- ￥2600
- Proteintech
- 0.156-10 ng/mL
- KE00498
- 2026年07月07日
企业认证
相关产品推荐更多 >
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 文献和实验
- 技术资料
- 检测范围：
0.156-10 ng/mL
- 应用：
Sandwich ELISA
- 适应物种：
Human
- 样本：
Cell lysate
- 规格：
96T
Product Information
KE00498 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human Beta Arrestin 2 ELISA kit is to be used to detect and quantify protein levels of endogenous human Beta Arrestin 2. The assay recognizes human Beta Arrestin 2. An antibody specific for human Beta Arrestin 2 has been pre-coated onto the microwells. The human Beta Arrestin 2 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human Beta Arrestin 2 is added to detect the captured human Beta Arrestin 2 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human Beta Arrestin 2 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Cell lysate 备注：对未验证样本，我们提供技术支持和售后保障。 |
| Assay type | Sandwich |
| Sensitivity | 0.04 ng/mL |
| Range | 0.156-10 ng/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 409 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Cell lysate | 91% | 86%-101% |
IntraAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 4.53 | 0.20 | 4.51 |
| 2 | 8 | 1.15 | 0.05 | 4.23 |
| 3 | 8 | 0.59 | 0.01 | 2.52 |
InterAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 4.75 | 0.32 | 6.82 |
| 2 | 16 | 1.15 | 0.05 | 4.02 |
| 3 | 16 | 0.58 | 0.02 | 3.24 |
Background Information
Beta - Arrestin 2 is a member of the arrestin family, which is involved in the regulation of G - protein - coupled receptor (GPCR) signaling. It is a cytosolic protein that is recruited to the plasma membrane upon GPCR activation. Beta - Arrestin 2 is involved in multiple antiproliferative pathways. It regulates the type III transforming growth factor beta (TGF - β) receptor and maternal embryonic leucine - zipper kinase (MELK), resulting in decreased TGF - β signaling and promoting the antiproliferative MELK pathway. eta - Arrestin 2 is involved in the pathogenesis of neurodegenerative diseases such as Parkinson's disease. It can affect the of accumulation alpha - synuclein and the function of dopaminergic neurons.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | ARRB2,Beta arrestin 2,β-Arrestin 2,ARB2,ARR2 |
FAQ
1、我的样本稀释液有限，如何处理稀释倍数较大的样本？
我们建议您对样本进行梯度稀释。例如，人血清样本需要1000000倍稀释时，您可以分3个梯度，每个梯度100倍，进行稀释：
第一步：取2 uL人血清样本，加入198 uL样本稀释液，混匀得到100倍稀释的人血清样本
第二步：取2uL 100倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到10000倍稀释的人血清样本
第三步：取2uL 10000倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到1000000倍稀释的人血清样本。
如果样本需要进一步稀释，您可以使用更多梯度。除了我们推荐的样本稀释液，不建议您使用其他溶液或者水稀释。如果您的样本稀释液仍然不足，请联系我们的客服为您提供更多样本稀释液。
风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验就是HEK293细胞系)。更高的转染效率是更高的病毒滴度的基本保障。 ViraPackTM 转染 试剂盒经Stratagene非常著名的MBS哺乳动物转染试剂盒改进而来，使用起来更为方便，推荐用于AdEasy® XL腺病毒载体系统，AAV(腺相关病毒)-无辅毒系统以及ViraPortTM反转录病毒系统，也可用于慢病毒载体。 ViraPackTM 病毒转染 试剂盒：货号#200488 试剂盒提供：2.5 M CaCl2, Solution，2x BBS (pH
Antigen Retrieval : for immunohistochemistry (Mike A. Dyer) Dissociated Cell Staining : immunohistochemistry (Mike A. Dyer) 相关链接： 仪器库 >> 显微系统 >> 倒置显微镜 仪器库 >> 影像系统 >>专业影像系统 试剂库 >> 免疫检测 >>ELISA检测试剂盒 抗体库 >> 抗体 抗体
国产抗体。当然我不否认咱们国产抗体也有好的抗体。做Western Blot， 就一个beta-actin内参抗体就需要几千了吧，还不包括AKT的一抗，二抗，转印膜， 蛋白定量试剂盒，显影试剂盒，医学胶片之类的试剂或耗材。 当然愿意累死累活天天跑电泳，过夜转膜的话，做Western blot是没有问题。我做过很长一段时间的western， 想想实在不是还在学校时期的学生能轻松胜任的，加上学校的条件还没有中科院好。 做Elisa 检测PI3K/AKT
技术资料暂无技术资料 索取技术资料
