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- 文献和实验
- 技术资料
- 检测范围:
1.95-125 pg/mL
- 应用:
Sandwich ELISA
- 适应物种:
Mouse/Rat
- 样本:
Mouse serum, Mouse plasma, Rat serum, Rat plasma, Cell culture supernatant, Mouse tissue lysate, Rat tissue lysate
- 规格:
96T
Product Information
KE20037 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The mouse/rat IGF1 ELISA kit is to be used to detect and quantify protein levels of endogenous mouse/rat IGF1. The assay recognizes mouse/rat IGF1. An antibody specific for mouse/rat IGF1 has been pre-coated onto the microwells. The mouse/rat IGF1 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for mouse/rat IGF1 is added to detect the captured mouse/rat IGF1 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Mouse/Rat IGF1 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Mouse serum, Mouse plasma, Rat serum, Rat plasma, Cell culture supernatant, Mouse tissue lysate, Rat tissue lysate 备注:对未验证样本,我们提供技术支持和售后保障。 |
| Assay type | Sandwich |
| Sensitivity | 0.2 pg/mL |
| Range | 1.95-125 pg/mL |
| Reactivity | Mouse/Rat |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 24482 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Mouse serum | 99% | 93%-106% |
| Rat serum | 89% | 87%-91% |
| Mouse tissue lysate | 95% | 81%-105% |
| Rat tissue lysate | 98% | 95%-101% |
| Cell culture supernatant | 98% | 86%-117% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 60.8 | 2.2 | 3.6 |
| 2 | 8 | 16.0 | 0.4 | 2.4 |
| 3 | 8 | 8.2 | 0.2 | 2.0 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 62.6 | 2.8 | 4.5 |
| 2 | 16 | 16.5 | 0.7 | 4.0 |
| 3 | 16 | 8.4 | 0.3 | 3.8 |
Background Information
Insulin-like-growth factor 1 (IGF1), a 70 amino-acid peptide hormone, is the principal mediator of biochemical effects of growth hormone (GH) and important in the regulation of cell proliferation and differentiation. IGF1 is largely synthesized in the liver (75%) and, to a lesser extent, in peripheral tissues. IGF1 is a potent mitogen and is inhibited by IGF-binding protein-3 (IGFBP3). High serum IGF1 and low IGFBP3 are associated with increased risk of several carcinomas. Mice lacking IGF1 exhibit generalized organ hypoplasia including underdevelopment of the central nervous system and developmental defects in bone, muscle and reproductive systems.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | Igf-1, IGF-I, Insulin-like growth factor I, Somatomedin |
FAQ
1、我的样本稀释液有限,如何处理稀释倍数较大的样本?
我们建议您对样本进行梯度稀释。例如,人血清样本需要1000000倍稀释时,您可以分3个梯度,每个梯度100倍,进行稀释:
第一步:取2 uL人血清样本,加入198 uL样本稀释液,混匀得到100倍稀释的人血清样本
第二步:取2uL 100倍稀释的人血清样本+ 198 uL样本稀释液进行稀释,混匀得到10000倍稀释的人血清样本
第三步:取2uL 10000倍稀释的人血清样本+ 198 uL样本稀释液进行稀释,混匀得到1000000倍稀释的人血清样本。
如果样本需要进一步稀释,您可以使用更多梯度。除了我们推荐的样本稀释液,不建议您使用其他溶液或者水稀释。如果您的样本稀释液仍然不足,请联系我们的客服为您提供更多样本稀释液。
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文献和实验流式细胞术 主要产品及服务: · 流式细胞分析试剂: · 流式检测抗体 · 流式检测试剂盒 · 流式检测辅助试剂 细胞因子试剂盒: · 人细胞因子ELISA检测试剂盒 · 小鼠细胞因子ELISA检测试剂盒 · 大鼠细胞因子ELISA检测试剂盒 · 猪细胞因子ELISA检测试剂盒 客户定制服务: · 蛋白免疫原的制备 · 多肽合成与交联 · 多、单克隆抗体的制备 · 抗体生产、纯化
抗体的轻、重链嵌合基因共转染受体细胞 目前将重组子导入宿主细胞主要采用的方法有磷酸钙沉淀法、DEAE-葡聚糖街道法介导法、聚季铵盐介导法、原生质体融合法、电转染法以及微注射法。其中以电转染效率较高。 【材料和试剂】 (1) 小鼠骨髓瘤细胞系Sp2/0细胞或P3X63.Ag8.653细胞,或CHO细胞。 (2) 已构建的轻、重链嵌合基因质粒DNA。 (3) 脂质体。 (4) 电转
6.8 的 1 / 15 mol/L 磷酸盐缓冲液。二、 试验方法2.1 受试物固体受试物应溶解于适合的溶媒中,并稀释至适当浓度。液体受试物可直接使用或稀释至适当浓度使用。受试物应无菌现用现配,否则须确认储存不影响其稳定性。2.2 细胞可选用中国仓鼠肺细胞株 (V79、CHL) 或卵巢细胞株 (CHO)、小鼠淋巴瘤细胞株 (L5178Y)、人外周血淋巴细胞株 (如 TK6) 和原代培养细胞。推荐使用 CHL 或 L5178Y 细胞株。细胞在使用前应进行染色体数目稳定性和有无支原体污染的检查。汇智泰康体外微核试验试剂盒
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