- ￥2600
- Proteintech
- 0.47-30 ng/mL
- KE00818
- 2026年07月06日
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- 详细信息
- 文献和实验
- 技术资料
- 检测范围：
0.47-30 ng/mL
- 应用：
Sandwich ELISA
- 适应物种：
Human
- 样本：
Serum, Plasma, Cell culture supernatant, Urine, Saliva
- 规格：
96T
Product Information
KE00818 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human E-cadherin ELISA kit is to be used to detect and quantify protein levels of endogenous human E-cadherin. The assay recognizes human E-cadherin. An antibody specific for human E-cadherin has been pre-coated onto the microwells. The human E-cadherin protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human E-cadherin is added to detect the captured human E-cadherin protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human E-cadherin ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatant, Urine, Saliva 备注：对未验证样本，我们提供技术支持和售后保障。 |
| Assay type | Sandwich |
| Sensitivity | 0.17 ng/mL |
| Range | 0.47-30 ng/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 999 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human serum | 97% | 87%-106% |
| Urine | 105% | 101%-110% |
| Saliva | 79% | 73%-84% |
| Cell culture supernatant | 99% | 97%-103% |
IntraAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 14.87 | 0.49 | 3.30 |
| 2 | 8 | 3.91 | 0.12 | 3.00 |
| 3 | 8 | 2.20 | 0.08 | 3.77 |
InterAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 14.02 | 1.11 | 7.90 |
| 2 | 16 | 3.69 | 0.30 | 8.03 |
| 3 | 16 | 2.10 | 0.13 | 6.33 |
Background Information
E-cadherin is a transmembrane glycoprotein that connects epithelial cells at adherens junctions. In normal cells, E-cadherin exerts its tumor-suppressing role mainly by sequestering β-catenin from its binding to LEF (Lymphoid enhancer factor)/TCF (T cell factor), which serves the function of transcribing genes of the proliferative Wnt signaling pathway. The loss of E-cadherin expression associated with the epithelial-mesenchymal transition (EMT) occurs frequently during tumor metastasis. Furthermore, Soluble E-cadherin (an 80-kDa soluble form) may be a biomarker for the diagnosis and prognosis of cancer.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | Arc 1, Cadherin 1, Cadherin-1, CAM 120/80, CD324 |
FAQ
1、我的样本稀释液有限，如何处理稀释倍数较大的样本？
我们建议您对样本进行梯度稀释。例如，人血清样本需要1000000倍稀释时，您可以分3个梯度，每个梯度100倍，进行稀释：
第一步：取2 uL人血清样本，加入198 uL样本稀释液，混匀得到100倍稀释的人血清样本
第二步：取2uL 100倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到10000倍稀释的人血清样本
第三步：取2uL 10000倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到1000000倍稀释的人血清样本。
如果样本需要进一步稀释，您可以使用更多梯度。除了我们推荐的样本稀释液，不建议您使用其他溶液或者水稀释。如果您的样本稀释液仍然不足，请联系我们的客服为您提供更多样本稀释液。
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文献和实验葡萄糖来源 AGEs ELISA 检测试剂盒 （ Glucose-derived AGEs ELISA Kit ） 研 究 背 景 越来越多的证据指出晚期糖基化终端产物（ AGEs ）修饰蛋白严重影响到老龄化人群的健康问题。 AGEs 是葡萄糖与自由的蛋白质氨基基团发生非酶促糖基化反应的终端不可逆代谢产物的总称。其在人体内的积累会引发组织或器官一系列的病理性变化，从而导致白内障、阿尔茨海默氏病、骨关节炎、心功
特异性。需要用不同的抗原和酶联物来检测相应的抗体，不可相互“通用” 。因此试剂生产成本较高。 其他应用： · ELISA：采用双抗夹心ELISA检测HSV1。 · 免疫胶体金：利用免疫层析原理制备免疫胶体金试纸条来检测HSV1。 · 免疫荧光：采用自制荧光标记HSV1 McAb以直接免疫荧光法(DFA)检测HSV1，以期可获得一种用于临床应用的快速检测HSV抗原的方法。 八、四正柏生物简介 北京四正柏生物科技有限公司（Beijing
。6）滴加正常山羊血清封闭液，室温20分钟。甩去多余液体。7）滴加 Ⅰ 抗,室温1小时或者4℃过夜或者37℃1小时（4℃过夜后在37℃复温45分钟）。8）PBS洗三次每次2分钟。9）滴加生物素化二抗,20℃～37℃ 20 分钟。10）PBC 洗 3 次每次 2 分钟。11）滴加试剂SABC，20℃～37℃ 20 分钟。12）PBS 洗 4 次每次 5 分钟。13）DAB 显色：DAB 显色试剂盒或者自配显色剂显色（镜下掌握显色程度）。14）蒸馏水洗。苏木素复染 2 分钟、盐酸酒精分化。15）脱水、透明
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