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文献和实验Detection of apoptotic process in situ using immunocytochemical and TUNEL assays
and go directly to step 4. B.3.2 Anticipated results To perform each experiment using a blank, a negative and a positive control samples, is recommended. The blank sample is assessed passing from step 14 directly to step 17, in order
were labeled with YO-PRO-1 dye (green) (Y3603). Necrotic cells were detected with propidium iodide (red) 10 µM喜树碱诱导的Jurkat cells（慢淋。G1检测点缺陷细胞） 凋亡。细胞然后经 Vybrant Apoptosis Assay Kit #4 (V13243).处理。凋亡细胞核被YO-PRO-1绿染，坏死细胞被pi红染。 Jurkat human T-cell leukemia
should be analyzed. Protein levels can be monitored by Western blot, immunofluorescence, ELISA or other means. (Protein can be isolated using Ambion's PARIS™ Kit.) For quantitating mRNA levels, however, qRT-PCR is again the preferred choice.An example
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