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- 2025年12月15日
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文献和实验Yeast IF with Methanol/Acetone Dehydration
This is the protocol to use with the Botstein anti-actin rabbit antibodies. Grow cells at the appropriate temperature to 5x10E6 in 5 mls YPD. Add 0.5mls 37 formaldehyde (best grade) and incubate on the roller at same temp. for 10
PREPARATION OF 2% FORMALDEHYDE STOCK SOLUTION (2 METHODS)
ml * 10% formaldehyde solution (e.g., Polysciences, Warrington, PA, ultrapure, Cat.#04018), depolymerized paraformaldehyde, EM grade, methanol-free solution.
Yeast IF with Methanol/Acetone Dehydration
Yeast IF with Methanol/Acetone Dehydration David Amberg Note this protocol is a modified version of the protocol from Mark Rose in the CSH Yeast Genetics Course Manual. This is the protocol to use with the Botstein anti-actin
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