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文献和实验Measurement with the Light Microscope
the focus. Any ocular scale must be calibrated, using a device called a stage micrometer. A stage micrometer is simply a microscope slide with a scale etched on the surface. A typical micrometer scale is 2 mm long and at least part of it should be etched
A. Plating: To sterilize glass coverslips, dip in ethanol and flame. We use 22x22x1 mm3 coverslips and put them in 6-well plates. Seed 100,000 cells per well overnight and fix the next day. B. Fixation: Remove the media
In Situ Hybridization Using Digoxigenin Labeled Probes
when disposing of unused diaminobenzidine and contaminated materials. 8) Place the slides horizontally in a tray and add 50 µl of staining solution onto each slide, cover with a 22 x 40 mm coverslip and incubate at room temperature for 1 - 10 minutes
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