- ￥206 - 6288
- TargetMol
- T2644
- 2026年07月07日
2 年钻石会员
企业认证
相关产品推荐更多 >
万千商家帮你免费找货
0 人在求购买到急需产品
相关阅读
源叶生物 十万种现货试剂速发，品质稳、品类全，实验不再等！Takara 秋促来袭，提取+反转录+定量套装仅 1200 元abinScience 体内级抗体 全年锁价 低至 ￥1200阿拉丁 22万+ 科研级产品现货速发，品质严控、科研无忧- 详细信息
- 文献和实验
- 技术资料
- CAS号：
658084-64-1
- 规格：
1mg/1mLx10mM(inDMSO)/2mg/5mg/10mg/25mg/50mg/100mg/500mg
| 规格： | 1mg | 产品价格： | ￥206.0 |
|---|---|---|---|
| 规格： | 1mLx10mM(inDMSO) | 产品价格： | ￥472.0 |
| 规格： | 2mg | 产品价格： | ￥284.0 |
| 规格： | 5mg | 产品价格： | ￥438.0 |
| 规格： | 10mg | 产品价格： | ￥789.0 |
| 规格： | 25mg | 产品价格： | ￥1272.0 |
| 规格： | 50mg | 产品价格： | ￥1944.0 |
| 规格： | 100mg | 产品价格： | ￥2920.0 |
| 规格： | 500mg | 产品价格： | ￥6288.0 |
Product Introduction
Bioactivity
| 名称 | (E)-Daporinad |
| 描述 | (E)-Daporinad (FK866) is a highly specific, non-competitive small molecule inhibitor of nicotinamide phosphoribosyltransferase (NAMPT) with potential anti-tumor and anti-angiogenic activities with an IC50 value of 0.09 nM. |
| 细胞实验 | For MTT assays, 0.5 × 106 cells/mL is plated in triplicate on 96-well plates. APO866 (0.01 nM-100 nM) is added in 50 μL of culture medium, with culture medium alone serving as control. After 72 or 96 hours of incubation, 15 μL of dye solution is added to each well and cells are incubated for an additional 4 hours. Stop solution (100 μL/well) is added for 1 hour and the absorbance is read at 570 nm on a spectrophotometer. For trypan blue dye exclusion staining, 0.5 × 105 cells/well is grown in 6-well plates with 1 mL media in the absence or presence of APO866 for 96 hours. Cells from each sample are incubated with 10 μL trypan blue solution (at a 1:1 ratio [vol/vol] for 1 minute). Cell survival is determined by calculating proportion of live (unstained) cells. (Only for Reference) |
| 激酶实验 | High Throughput Screening: FITC-MBM1 at 15 nM and menin at 150 nM in the FP buffer are mixed and incubated for 1h in the dark at room temperature. For point screening, the 0.2 μL of each compound (20 μM final concentration, 1% DMSO) is added to 20 μL of the aliquot of the protein-peptide mixture and incubated on 384-well plates in the dark at room temperature for 1h. In confirmation screening, the serial dilution plates with compounds in DMSO are prepared and used to titrate the menin-FITC-MBM1 complex. Change in fluorescence polarization is monitored at 525 nm after excitations at 495 nm using the PHERAstar microplate reader (BMG) and applied to determine IC50 values with the Origin 7.0 program. |
| 体外活性 | 方法：用 (E)-Daporinad（ FK866 ）（100 nM，30 分钟） 预处理肝细胞，然后进行 GaIN/LPS（G/L，1 mg/mL /30 ng/mL，24 小时）攻击，观察(E)-Daporinad（ FK866 ）是否能减轻GaIN/LPS诱导的肝毒性。 结果：用(E)-Daporinad（ FK866 ）预处理的原代肝细胞在GaIN/LPS损伤后表现出自噬活性显着增加，(E)-Daporinad（ FK866 ）可以改善GaIN/LPS和ConA诱导的肝损伤，其保护机制可能涉及其通过抑制JNK诱导自噬的能力。[1] |
| 体内活性 | 方法：用GaIN/LPS和ConA处理小鼠前24、12和0.5小时施用(E)-Daporinad（ FK866 ）（10mg / kg，腹腔注射），测试(E)-Daporinad（ FK866 ）对小鼠ALF的潜在影响。 结果：(E)-Daporinad（ FK866 ）治疗后降低了GaIN/LPS或ConA治疗小鼠的死亡率，(E)-Daporinad（ FK866 ）处理前和处理后均能减弱小鼠GaIN/LPS和ConA诱导的ALF，且(E)-Daporinad（ FK866 ）预处理对GaIN/LPS或ConA攻击的响应效果更好。[1] |
| 存储条件 | Store at low temperature Powder: -20°C for 3 years | In solvent: -80°C for 1 year Shipping with blue ice/Shipping at ambient temperature. |
| 溶解度 | DMSO : 257.5 mg/mL (657.71 mM), Sonication is recommended. 10% DMSO+90% Saline : 0.1 mg/mL (0.26 mM), Solution. Ethanol : 72 mg/mL (183.9 mM), Sonication is recommended. |
| 关键字 | Visfatin | Pre-B cell colony enhancing factor | PBEF | NMPRTase | Nicotinamide phosphoribosyl transferase | NAMPT | Inhibitor | inhibit | FK-866 | FK 866 | Autophagy | APO-866 | APO 866 | (E)-Daporinad | (E)Daporinad | (E) Daporinad |
| 相关产品 | Guanidine hydrochloride | Naringin | Aceglutamide | Alginic acid | Cysteamine hydrochloride | Hemin | Hydroxychloroquine | Sildenafil citrate | Stavudine | Tamoxifen | Paeonol | D(+)-Raffinose pentahydrate |
| 相关库 | Inhibitor Library | Anti-Cancer Active Compound Library | Bioactive Compound Library | Bioactive Compounds Library Max | Metabolism Compound Library | Anti-Aging Compound Library | Anti-COVID-19 Compound Library | Glycometabolism Compound Library | Drug Repurposing Compound Library | Anti-Cancer Clinical Compound Library | Target-Focused Phenotypic Screening Library | Anti-Cancer Drug Library |
风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验该产品被引用文献
官网有文献
技术资料暂无技术资料 索取技术资料
文献支持
达珀利奈【658084-64-1】
￥206 - 6288
