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- 文献和实验
- 技术资料
- CAS号:
36330-85-5
- 规格:
1g/1mLx10mM(inDMSO)/50mg/100mg/500mg
| 规格: | 1g | 产品价格: | ¥430.0 |
|---|---|---|---|
| 规格: | 1mLx10mM(inDMSO) | 产品价格: | ¥212.0 |
| 规格: | 50mg | 产品价格: | ¥144.0 |
| 规格: | 100mg | 产品价格: | ¥194.0 |
| 规格: | 500mg | 产品价格: | ¥330.0 |
Product Introduction
Bioactivity
| 名称 | Fenbufen |
| 描述 | Fenbufen (Lederfen) is a non-steroidal anti-inflammatory drug used primarily to treat inflammation in osteoarthritis, ankylosing spondylitis, and tendinitis. It can also be used to relieve backaches, sprains, and fractures. Fenbufen is available as a capsule or tablet sold with the brand names Cepal, Cinopal, Cybufen, Lederfen, and Reugast. Fenbufen acts by preventing cyclooxygenase from producing prostaglandins which can cause inflammation. |
| 体内活性 | Fenbufen and its metabolites could be involved in mitochondrial toxicity through inhibition of ATP synthesis[1]. Fenbufen inhibited prostaglandin release by 80% and reduced rates of protein synthesis in normal muscle and in muscle undergoing hypertrophy in response to tenotomy of a synergist[3]. |
| 存储条件 | Powder: -20°C for 3 years | In solvent: -80°C for 1 year Shipping with blue ice/Shipping at ambient temperature. |
| 溶解度 | 10% DMSO+90% Corn oil : 10 mg/mL (39.33 mM), Solution. Ethanol : 47 mg/mL (184.84 mM), Sonication is recommended. DMSO : 250 mg/mL (983.17 mM), Sonication is recommended. 10% DMSO+40% PEG300+5% Tween 80+45% Saline : < 10 mg/mL (39.33 mM), Lower concentrations may be soluble, but exact solubility limit is unknown. 10% DMSO+90% (20% SBE-β-CD in Saline) : < 10 mg/mL (39.33 mM), Lower concentrations may be soluble, but exact solubility limit is unknown. H2O : < 1 mg/mL (insoluble or slightly soluble) 10% DMSO+90% Corn Oil : 2 mg/mL (7.87 mM), Sonication is recommended. 10% DMSO+90% Saline : < 10 mg/mL (39.33 mM), Lower concentrations may be soluble, but exact solubility limit is unknown. |
| 关键字 | tendinitis | osteoarthritis | Inhibitor | inhibit | Fenbufen | Cyclooxygenase | COX | CL82204 | CL 82204 | Caspase | ATP systhesis | antirheumatic | antipyretic | anti-inflammatory | ankylosing spondylitis | analgetic |
| 相关产品 | Trometamol | Sodium orthovanadate | Magnesium acetate tetrahydrate | Gallic Acid Monohydrate | 5-Methylfurfural | Indomethacin | Paradol | Acetaminophen | Cystamine dihydrochloride | Copper Sulfate Pentahydrate | Magnesium sulfate | Phenidone |
| 相关库 | Inhibitor Library | Anti-Cancer Approved Drug Library | Bioactive Compound Library | Bioactive Compounds Library Max | Anti-Aging Compound Library | Protease Inhibitor Library | Membrane Protein-targeted Compound Library | Immunology/Inflammation Compound Library | Orally Active Compound Library | Pain-Related Compound Library | Drug Repurposing Compound Library | Anti-Cancer Drug Library |
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文献和实验的假复层上皮结构。随着时间的推移,培养 60-85 天的类器官状态最佳。 B、生成芽尖祖细胞 11B、向各孔中加入 0.5ml 人芽尖祖细胞类器官培养基,确保完全覆盖 Matrigel 液滴。每 3-5 天换液一次。 12B、如果类器官出现腔中积攒了细胞碎片,或下沉到 Matrigel 的底部,则用移液管轻轻刮培养孔底部,以解离含有类器官的 Matrigel。 13B、用移液器将 Matrigel 液滴和周围的培养基一同吸出,并放置于显微镜下,轻轻去除旧的 Matrigel,小心不要切到组织
实验原理 免疫电泳又称为γ球蛋白电泳或免疫球蛋白电泳技术,这是一种能够判断血液中三种免疫球蛋白IgM,IgG,IgA含量水平的实验方法。在这项实验技术中,首先利用蛋白质的分子量和所带电荷的比值运用水平琼脂糖凝胶电泳技术将蛋白质进行分离,然后将特异性的抗体引入到与电泳方向平行的凹槽中,在抗原和抗体的扩散过程中,抗原和抗体适当比例的结合会导致沉淀的产生。0.85%盐不仅可以终止扩散过程也可用于洗去未结合的蛋白,抗原和抗体结合所形成
的 6 孔板中,每孔加入 3ml mTeSR1,放置在 37℃,5%CO2 的培养箱中,培养至细胞融合度约为 75–85%、且大部分无分化的状态时,吸出培养基。 3、向 hPSCs 中加入 1 ml Dispase(1mg/ml),并将培养皿放置在 37℃ 条件下进行解离,直到所有的细胞都以小细胞块或单细胞的形式漂浮。向每个孔中加入 5ml的DMEM-F12,以充分稀释 Dispase。 4、将所有细胞转移至离心管中,室温下 300g 离心 3min,收集沉淀细胞。加入 DMEM-F12 培养
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