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- CAS号：
17692-71-6
- 规格：
1mLx10mM(inDMSO)/10mg/25mg/50mg/100mg/200mg
| 规格： | 1mLx10mM(inDMSO) | 产品价格： | ￥196.0 |
|---|---|---|---|
| 规格： | 10mg | 产品价格： | ￥276.0 |
| 规格： | 25mg | 产品价格： | ￥420.0 |
| 规格： | 50mg | 产品价格： | ￥704.0 |
| 规格： | 100mg | 产品价格： | ￥1104.0 |
| 规格： | 200mg | 产品价格： | ￥1744.0 |
Product Introduction
Bioactivity
| 名称 | Vanitiolide |
| 描述 | Vanitiolide (Vanitiolid) has cholelitic effects. |
| 激酶实验 | DPP-4 is extracted from confluent Caco-2 cells. After 5 minutes of incubation at room temperature with lysis buffer (10 mM Tris-HCl, 150 mM NaCl, 0.04 U/mL aprotinin, 0.5% Nonidet P40, pH 8.0), cells are centrifuged at 35,000 g at 4°C for 30 minutes, and the supernatant is stored at -80°C. Assays are performed by mixing 20 μL of appropriate compound dilutions with 50 μL of the substrate for the DPP-4 enzyme, H-Ala-Pro-7-amido-4-trifluoromethylcoumarin (final concentration in the assay, 100 μM) and 30 μL of the Caco-2 cell extract (diluted 1000-fold with 100 mM Tris-HCl, 100 mM NaCl, pH 7.8). Plates are incubated at room temperature for 1 hour, and fluorescence is measured at excitation/emission wavelengths of 405/535 nm using a SpectraMax GeminiXS. Dissociation kinetics of inhibitors from the DPP-4 enzyme is determined after a 1-hour preincubation of Caco-2 cell extracts with high inhibitor concentrations (30 nM for BI 1356, 3 μM for vildagliptin). The enzymatic reaction is started by adding the substrate H-Ala-Pro-7-amido-4-trifluoromethylcoumarin after a 3000-fold dilution of the preincubation mixture with assay buffer. Under these conditions, the difference in DPP-4 activity at a certain time point in the presence or absence of an inhibitor reflects the amount of this inhibitor still bound to the DPP-4 enzyme. Maximal reaction rates (fluorescence units/seconds ×1000) at 10-minute intervals are calculated using the SoftMax software of the SpectraMax and corrected for the rate of an uninhibited reaction [(vcontrol-vinhibitor)/vcontrol]. |
| 存储条件 | Powder: -20°C for 3 years | In solvent: -80°C for 1 year Shipping with blue ice/Shipping at ambient temperature. |
| 溶解度 | DMSO : 9 mg/mL (35.53 mM), Sonication is recommended. |
| 关键字 | Vanitiolide | Inhibitor | inhibit |
| 相关库 | Bioactive Compound Library | Bioactive Compounds Library Max | NO PAINS Compound Library | Natural Product Library | Natural Product Library for HTS | RO5 Drug-like Natural Product Library |
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￥196 - 1744
