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- 详细信息
- 文献和实验
- 技术资料
- 形态:
Liquid
- 保存条件:
4°C
- 克隆性:
单克隆
- 标记物:
PE
- 适应物种:
Human
- 保质期:
12 months
- 级别:
一抗
- 供应商:
武汉研升生物科技有限公司
- 宿主:
Mouse
- 应用范围:
FCM
- 抗体英文名:
Anti-Human CD16b/FCGR3B Antibody (B88-9), PE
- 规格:
50T/100T
| 规格: | 50T | 产品价格: | ¥1740.0 |
|---|---|---|---|
| 规格: | 100T | 产品价格: | ¥2892.0 |
| Product name | Anti-Human CD16b/FCGR3B Antibody (B88-9), PE |
| Catalog No. | HF886127 |
| Target | IGFR3, FCGR3B, CD16b, Low affinity immunoglobulin gamma Fc region receptor III-B, Fc-gamma RIII, IgG Fc receptor III-1, FCGR3, CD16B, Fc-gamma RIII-beta, Fc-gamma RIIIb, FCG3, FcRIII, FcR-10, FcRIIIb |
| Clone ID | B88-9 |
| Accession | O75015 |
| Clonality | Monoclonal |
| Isotype | IgG1, kappa |
| Host species | Mouse |
| Species reactivity | Human |
| Applications | FCM |
| Conjugation | PE |
| Form | Liquid |
| Storage buffer | 0.01M PBS, pH 7.4, 0.2% BSA, 0.05% Proclin 300. |
| Stability and Storage | Store at 4°C for 12 months. Protect from light. Do not freeze. |
| Note | For flow cytometric staining, the suggested use of this reagent is 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application. For research use only. |

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文献和实验Western Blotting with Horseradish Pe
. 3.Centrifuge the homogenate (16,000 x g, 15 C) for 5 minutes to pellet insoluble material, then discard pellet. 4.Dilute an aliquot of the tissue lysate sample at least 10-fold for the BCA (Pierce) protein concentration assay. POLYACRYLAMIDE GEL
attractive not only for studying various aspect of BCR signaling but also for the generation of human monoclonal antibody and the analysis of the human B cell repertoire. This chapter describes an efficient method to immortalize and clone human B cells
T-cell dependent antigen specific in situ staining (Pe)
and then apply 3% human serum as a blocking agent (health hazard!). 4- incubate with the blocking for 10 min. 5- blot the slides without washing and apply Phycoerythrin (B-Pe or R-Pe) 0.5µg/ml in PBS-BSA-NaN 3 , in a moist chamber, at RT for 1-18 hr.
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