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- 文献和实验
- 技术资料
- 保存条件:
4℃
- 保质期:
12 months
- 供应商:
武汉益普生物科技有限公司
- 规格:
50T/100T
| 规格: | 50T | 产品价格: | ¥1740.0 |
|---|---|---|---|
| 规格: | 100T | 产品价格: | ¥2892.0 |
| 产品名称 | Anti-Human HLA-A, B, C Antibody (W6/32), PE |
| 货号 | HM834127 |
| 描述 | Anti-Human HLA-A, B, C Antibody (W6/32), PE [W6/32] ( HM834127 ) is a mouse monoclonal antibody in ELISA, FCM, ICC-Fr, WB . Suitable for Human . Highlights Multi-Application — Validated across multiple applications. Flow Cytometry — Validated for flow cytometry applications. |
| 反应种属 | Human |
| 应用实验 | ELISA, FCM, ICC-Fr, WB |
| 宿主/表达体系 | Mouse |
| 状态 | Liquid |
| 储存温度 | 4℃ |

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文献和实验A/B/C Random Amplification Protocol
the Round B PCR program and place the samples on the machine when it reaches 94˚C. Part B -- PCR machine parameters: (94˚C 3’) x1 (94˚C 30”, 40˚C 30”, 50˚C 30”, 72˚C 1’) x35 cycles (4ºC hold) x1 3. To check if the PCR reaction went
Western Blotting with Horseradish Pe
. 3.Centrifuge the homogenate (16,000 x g, 15 C) for 5 minutes to pellet insoluble material, then discard pellet. 4.Dilute an aliquot of the tissue lysate sample at least 10-fold for the BCA (Pierce) protein concentration assay. POLYACRYLAMIDE GEL
T-cell dependent antigen specific in situ staining (Pe)
and then apply 3% human serum as a blocking agent (health hazard!). 4- incubate with the blocking for 10 min. 5- blot the slides without washing and apply Phycoerythrin (B-Pe or R-Pe) 0.5µg/ml in PBS-BSA-NaN 3 , in a moist chamber, at RT for 1-18 hr.
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