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- 详细信息
- 文献和实验
- 技术资料
- 供应商:
上海阿拉丁生化科技股份有限公司
- 库存:
现货
- 免疫原:
Human IgG
- 浓度:
0.5mg/mL
- 保存条件:
2-8°C储存,避光,禁止冷冻;低温运输,禁止冷冻
- 规格:
Ab226757-100μg/Ab226757-10μg/Ab226757-500μg
| 规格: | Ab226757-100μg | 产品价格: | ¥699.90 |
|---|---|---|---|
| 规格: | Ab226757-10μg | 产品价格: | ¥169.90 |
| 规格: | Ab226757-500μg | 产品价格: | ¥1999.90 |
基本信息
产品名称:Mouse Anti-Human IgG (PerCP-Cy5.5)
规格或纯度:ExactAb™, 已验证, 0.5mg/mL
应用:Flow Cytometry
特异性:Human IgG
种属反应性:人(Human)
交叉反应:No cross reactivity with mouse IgG, rabbit IgG and goat IgG.
免疫原:Human IgG
偶联:PerCP-Cy5.5
产品属性
克隆类型:单克隆抗体
Format:Whole IgG
轻链亚型:kappa
纯化方法:Octylic acid-ammonium sulfate precipitation
储存缓冲液:Liquid in PBS pH 7.4, containing 1% BSA and 0.05% Proclin300.
防腐剂:0.05% Proclin300
浓度:0.5mg/mL
储存条件:2-8°C储存,避光,禁止冷冻
运输条件:低温运输,禁止冷冻
稳定性与储存:The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
功能和特点
宿主种属:小鼠(Mouse)
种属反应性:人(Human)
克隆号:1H8
偶联:PerCP-Cy5.5
图片
Mouse Anti-Human IgG (PerCP-Cy5.5) (Ab226757) - Flow Cytometry
Two-color flow cytometric analysis of IgG expression on viable Human peripheral blood lymphocytes. Cells were preincubated with Human Fc Receptor Blocking Solution (H1374078). The cells were then stained with Recombinant CD19 Antibody (AF405) (Ab223516) and either Mouse IgG (PerCP-Cy5.5) (Ab190081; Left panel) or Mouse Anti-Human IgG (PerCP-Cy5.5) (Ab226757; Right panel) at 0.3μg/test.
Mouse Anti-Human IgG (PerCP-Cy5.5) (Ab226757) - Flow Cytometry
Flow Cytometry analysis of HT-29 cells labelling EpCAM (red) with Citatuzumab (anti-EpCAM) (Ab209902). Mouse Anti-Human IgG (PerCP-Cy5.5) (Ab226757) at a dilution of 1/500 was used as the secondary antibody. Blue - Isotype control, Human IgG (Ab170213). Black - Unlabelled control, cells without incubation with primary antibody.
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文献和实验-purified goat anti-mouse IgG (Jackson Labs; 1 mg/ml stock used at 1:300 dilution) Texas Red-conjugated affinity-purified donkey anti-rabbit (Jackson Labs;1 mg/ml stock used at 1:200 dilution)
at 1:200 dilution) Cy3-conjugated affinity-purified goat anti-mouse IgG (Jackson Labs; 1 mg/ml stock used at 1:300 dilution) Texas Red-conjugated affinity-purified donkey anti-rabbit (Jackson Labs;1 mg/ml stock used at 1:200 dilution)
buffer (pH9.0) ~ 200 ml. Filter protein mixture Add protein mixture or culture supernatant containing Ab (adjust pH to 7.8 with Binding buffer ; red color) to the Protein A column. Mouse antibodies of the IgG1 subclass
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