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- 详细信息
- 文献和实验
- 技术资料
- 库存:
期货
- 规格:
M1007492-1mg
中文名:Mci 826
英文名:Mci 826
货号:M1007492
Cas号:140646-80-6
存储温度:室温
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文献和实验相关专题 (一)材料及试剂 1.培养基 RPMI-1640 或TC199培养液 2.PHA 同形态学方法 3.小牛血清 4.3H-TdR 选用比活性为2Mci~10Mci/mg分子 的制品,将1 Mci/ml溶液以生理盐水稀释成100µci/ml,于4℃保存备用。临用时再用培养液稀释成10µci/ml溶液。 5.3%冰醋酸 6.浓甲醛 7.30%H2O2液 8.闪烁液 PPO(2,5二苯基噁唑
Exposing gels and plates containing radioactive samples to X-ray film
below which nothing can be detected. Flashing reduces this threshold. Detection of 3H. 3H cannot be detected by autoradiography unless you have 1 mCi of it in your band. Because this is almost unheard of, it is essential to impregnate a gel containing 3H
嘧啶核苷(3H-TdR)掺入率测定T细胞的增殖水平。二、仪器、材料及试剂1、Beckman LS-9800型液体闪烁计数仪,或Beckman LS 6500型液体闪烁计数仪。2、多头细胞收集仪。3、3H-TdR(比活性为2Mci~10Mci/mg分子)。3H-TdR工作液:将1Mci/ml溶液以生理盐水稀释成100μci/ml,于4℃保存备用。临用时再用培养液稀释成10μci/ml溶液。3H-TdR一般临用时稀释。4、闪烁液:PPO(2,5-二苯基恶唑)、POPOP(1,4-双-2-15-苯基恶唑苯
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