- ￥300 - 3000
- proteintech
- 国内
- 14292-1-AP
- 2026年05月11日
- WB, ELISA
- Rabbit / IgG
- human, mouse
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
详询
- 亚型：
IgG
- 形态：
液体
- 保存条件：
-20℃
- 克隆性：
Polyclonal
- 标记物：
Unconjugated
- 适应物种：
human, mouse
- 保质期：
详询
- 抗原来源：
详询
- 目录编号：
详询
- 供应商：
上海艾科斯生物科技有限公司
- 宿主：
Rabbit / IgG
- 应用范围：
WB, ELISA
- 浓度：
400 ug/ml
- 靶点：
CD80
- 抗体英文名：
CD80
- 抗体名：
CD80
- 规格：
50 μL/100 μL/150 μL
【产品概述】
本产品为CD80 Polyclonal antibody（货号：14292-1-AP），由Rabbit / IgG宿主制备。该抗体属于Polyclonal抗体，可识别human, mouse来源的靶蛋白，经测试适用于WB, ELISA等实验应用。产品形式为Liquid，偶联物类型为Unconjugated。
【背景介绍】
CD80 (also known as B7-1) is a type I membrane protein that is a member of the immunoglobulin superfamily, with an extracellular immunoglobulin constant-like domain and a variable-like domain required for receptor binding. It is expressed on antigen-presenting cells (APCs), including B cells, dendritic cells, monocytes, and macrophages. CD80 is the receptor for the proteins CD28 and CTLA-4 found on the surface of T-cells. It is involved in the costimulatory signal essential for T-lymphocyte activation. T-cell proliferation and cytokine production is induced by the binding of CD28, binding to CTLA-4 has opposite effects and inhibits T-cell activation. CD80 also acts as a cellular attachment receptor for adenovirus subgroup B. (PMID: 7545666; 12015893; 16920215)
【产品特点】
本抗体采用Antigen affinity purification方式纯化，确保了高特异性和批次间一致性。观测分子量约为60 kDa。推荐稀释比：WB: WB : 1:500-1:2000；ELISA: See 1 publications below。Western Blot检测阳性对照组织/细胞为Raji cells, RAW 264.7 cells, human peripheral blood leukocyte, Ramos cells。储存缓冲液为PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.，储存条件为Store at -20°C. Stable for one year after shipment.Aliquoting is unnecessary for -20oC storage.。Proteintech抗体经过严格的质量验证流程，确保研究人员获得可重复且可靠的实验结果。
【产品信息】
GenBank蛋白编号：BC042665；基因名称：CD80；Gene ID (NCBI)：941；UNIPROT ID：P33681；RRID：AB_10640809；别名包括：Activation B7 1 antigen, Activation B7-1 antigen, B7, B7 1, B7-1；免疫原为CatNo:Ag5615Product name: Recombinant human B7-1 proteinSource:e coli.-derived, PGEX-4TTag: GSTDomain: 36-228 aa ofBC042665Sequence: IHVTKEVKEVATLSCGHNVSVEELAQTRIYWQKEKKMVLTMMSGDMNIWPEYKNRTIFDITNNLSIVILALRPSDEGTYECVVLKYEKDAFKREHLAEVTLSVKADFPTPSISDFEIPTSNIRRIICSTSGGFPEPHLSWLENGEELNAINTTVSQDPETELYAVSSKLDFNMTTNHSFMCLIKYGHLRVNQT种属同源性预测；储存缓冲液为PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.，储存条件为Store at -20°C. Stable for one year after shipment.Aliquoting is unnecessary for -20oC storage.；产品形式为Liquid；纯化方式为Antigen affinity purification。
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文献和实验Polyclonal Antibody Production
, if desired this is best accomplished by Protein A Affinity Chromatography. 上一篇：make an Antibody against a Peptides 下一篇：Immunization Protocol for Monoclonal Antibody Production
Polyclonal Antibody Production
Polyclonal Antibody Production Very useful for rapid and simple generation of antibodies for western blots, ELISA assays, and immunoprecipitation. Rabbit Immunization Initial Preparation Your antigen
Construction of Polyclonal Antibody Libraries Using Phage Display
Polyclonal antibody libraries (PCALs) are standardized mixtures of antibodies (Abs) specific for an antigen (Ag) or multi-Ag target (a poly-Ag). As the immunoglobulin (Ig) genes are cloned, the mixtures can be perpetuated, amplified
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