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- 详细信息
- 文献和实验
- 技术资料
- 库存:
100
- 供应商:
上海富雨
- 检测范围:
/
- 检测方法:
酶联免疫吸附试验法
- 应用:
检测
- 适应物种:
人
- 标记物:
HRP
- 样本:
血清、血浆、尿液、细胞培养上清、组织标本
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产品详情
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产品描述:人血管紧张素Ⅱ(ANG-Ⅱ)酶联免疫试剂盒(CSB-E04493h)为竞争法ELISA试剂盒,定量检测血清、血浆样本中的ANG-Ⅱ含量。ANG-Ⅱ即血管紧张素Ⅱ,在心血管系统调节中有着重要背景。它可引起血管收缩、醛固酮释放等。研究机制方面,其通过与特异性受体结合,激活多条信号通路,影响细胞生长、增殖,在高血压、心衰等疾病发病中扮演关键角色。试剂盒检测范围为31.25 pg/ml-2000 pg/ml.,适用于科研场景中探究ANG - Ⅱ在心血管疾病模型中的动态变化、药物干预效果评估或相关代谢调控机制分析,为心血管疾病基础研究、药理学实验及生物标志物探索提供可靠工具本品仅用于科研,不用于临床诊断,产品具体参数及操作步骤详见产品说明书。
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关联名:angiotensin Ⅱ, angiotensin 2, ANG2
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缩写:ANG-Ⅱ
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种属:Homo sapiens (Human)
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反应时间:1-5h
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上样量:50-100ul
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检测波长:450 nm
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研究领域:Cardiovascular
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测定原理:quantitative
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测定方法:Competitive
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精密度:
Intra-assay Precision (Precision within an assay): CV%<12%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<15%
Three samples of known concentration were tested in twenty assays to assess.
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线性度:
To assess the linearity of the assay, samples were spiked with high concentrations of human ANG- Ⅱ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
Sample
Serum(n=4)
1:1
Average %
91
Range %
85-101
1:2
Average %
89
Range %
82-95
1:4
Average %
93
Range %
88-99
1:8
Average %
96
Range %
89-103
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回收率:
The recovery of human ANG- Ⅱ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type
Average % Recovery
Range
Serum (n=5)
91
84-98
EDTA plasma (n=4)
87
81-95
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标准曲线:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml
OD1
OD2
Average
2000
0.159
0.154
0.157
1000
0.214
0.222
0.218
500
0.292
0.286
0.289
250
0.370
0.398
0.384
125
0.568
0.561
0.565
62.5
0.915
0.933
0.924
31.25
1.346
1.284
1.315
0
2.207
2.125
2.166
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本试剂盒所含材料:
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- A micro ELISA plate --- The 96-well plate has been pre-coated with an anti-human ANG-II antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
- Two vials lyophilized standard --- Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
- One vial Biotin-labeled ANG-II antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.
- One vial HRP-avidin (100 x concentrate) (120 μl/bottle) --- Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
- One vial Biotin-antibody Diluent (15 ml/bottle) ---Dilute the Biotin-antibody.
- One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.
试剂盒不含材料:-
- A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
- An incubator that can provide stable incubation conditions up to 37°C±5°C.
- Centrifuge
- Vortex
- Squirt bottle, manifold dispenser, or automated microplate washer
- Absorbent paper for blotting the microtiter plate
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文献和实验Analysis of the effect of PECAM1 on the migration and invasion of gastrointesti- nal stromal tumor cells based on single-cell transcriptome sequencing data
期刊:MODERN ONCOLOGY,Aug.2023,VOL. 31,No. 16
作者:HU Wu1,WEN Yiqiao2,QIN Jialan1,ZHAO Ying3,ZHANG Tianbiao1
DOI:10.3969 /j.issn.1672-4992.2023.16.003
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