JSH-23

JSH-23 functions as an NF-κB inhibitor, effectively suppressing NF-κB transcriptional activity with an IC50 of 7.1 μM in lipopolysaccharide (LPS)-stimulated macrophages RAW 264.7. It achieves this by impeding the nuclear translocation of NF-κB p65 without impacting IκBα degradation¹.

When administered at concentrations below 100 μM for 24 hours, JSH-2 does not induce significant cytotoxic effects on RAW 264.7 cells¹.

Upon exposure to LPS for 1 hour, the nuclear content of NF-κB p65 significantly increases. Treatment with JSH-23 (30 μM; 1 hour) reduces the nuclear content of NF-κB p65 in LPS-stimulated RAW 264.7 cells in a dose-dependent manner¹.

JSH-23 (1 mg/kg and 3 mg/kg; orally administered; daily; for 2 weeks) significantly mitigates nerve conduction and nerve blood flow deficits observed in diabetic rats².

JSH-23 (1 mg/kg and 3 mg/kg; orally administered; daily; for 2 weeks) significantly mitigates nerve conduction and nerve blood flow deficits observed in diabetic rats².

溶解方案(细胞实验)

DMSO 中的溶解度 : ≥ 56 mg/mL (233.00 mM; 吸湿的 DMSO 对产品的溶解度有显著影响，请使用新开封的 DMSO)|* "≥" means soluble, but saturation unknown.

溶解方案(动物实验)

"方案 一": "请依序添加每种溶剂：10% DMSO 40% PEG300 5% Tween-80 45% SalineSolubility: ≥ 2.5 mg/mL (10.40 mM); 澄清溶液 此方案可获得 ≥ 2.5 mg/mL（饱和度未知）的澄清溶液。以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中，混合均匀；再向上述体系中加入 50 μL Tween-80，混合均匀；然后再继续加入 450 μL 生理盐水 定容至 1 mL。生理盐水的配制：将 0.9 g 氯化钠，溶解于 ddH₂O 并定容至 100 mL，可以得到澄清透明的生理盐水溶液。"

"方案 二": "请依序添加每种溶剂：10% DMSO 90% Corn OilSolubility: ≥ 2.5 mg/mL (10.40 mM); 澄清溶液 此方案可获得 ≥ 2.5 mg/mL（饱和度未知）的澄清溶液，此方案实验周期在半个月以上的动物实验酌情使用。以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL玉米油中，混合均匀。"