- ￥1680 - 2400
- ELK Biotechnology
- 15.63-1000 pg/mL
- 6 pg/mL
- 武汉
- ELK9482
- 2026年05月21日
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- 详细信息
- 文献和实验
- 技术资料
- 供应商：
武汉研升生物科有限公司
- 检测范围：
15.63-1000 pg/mL
- 检测方法：
Sandwich
- 适应物种：
Human
- 样本：
Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 灵敏度：
6 pg/mL
- 规格：
48T/96T
| 规格： | 48T | 产品价格： | ￥1680.0 |
|---|---|---|---|
| 规格： | 96T | 产品价格： | ￥2400.0 |
| 中文名称 | 人神经酰胺合酶1(CERS1)酶联免疫吸附检测试剂盒 |
| 英文名称 | Human CERS1(Ceramide synthase 1) ELISA Kit |
| 别名 | Ceramide synthase 1; CerS1; CERS1_HUMAN; LAG1; LAG1 homolog ceramide synthase 1; LAG1 longevity assurance homolog 1; LASS 1; LASS1; Longevity assurance (LAG1 S. cerevisiae) homolog 1; Longevity assurance gene 1; Longevity assurance gene 1 protein homolog 1; MGC90349; Protein LAG1; Protein UOG 1; Protein UOG-1; UOG 1 protein; UOG1; Upstream of GDF1; |
| 货号 | ELK9482 |
| 反应种属 | Human |
| P27544 | P27544 |
| 检测类型 | Sandwich |
| 灵敏度 | 6 pg/mL |
| 标准品 | 1000 pg/mL |
| 检测范围 | 15.63-1000 pg/mL |
| 样本类型 | Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids |
| 反应时间 | 3.5h |
| 检测原理 | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human CERS1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human CERS1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human CERS1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human CERS1 in the samples is then determined by comparing the OD of the samples to the standard curve. |
| 研究领域 | Signal Transduction; Metabolism; Cancer |
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文献和实验相关实验
常用1%以下的中性去垢剂，如Tween、Triton、Lubel和 NP40等加EDTA和还原剂2-巯基苏糖醇（DTT）、β-巯基乙醇等反复多次进行，因去垢剂洗涤能力随溶液离子强度升高而加强，在洗涤包涵体时可加50 mM NaCL。这样提取的包涵体纯度至少可达50%以上，而且可保持元结构。也可用低浓度的盐酸胍或尿素/中性去垢剂/EDTA/还原剂等洗去包涵体表面吸附的大部分不溶性杂蛋白。洗涤液pH以与工程菌生理条件相近为宜，使用的还原剂为0.1-5mM。EDTA为0.1-0.3 mM。去垢
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人神经酰胺合酶1(CERS1)酶联免疫吸附检测试剂盒【Human CERS1(Ceramide synthase 1) ELISA Kit】
￥1680 - 2400
